pLKO.1-puro

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pLKO.1-puro

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pLKO.1-puro

pLK0.1-puro载体基本信息

出品公司:	Ybscience
载体名称:	pLKO.1 puro,  pLK0.1 puro
质粒类型:	慢病毒载体；RNAi载体
高拷贝/低拷贝:	高拷贝
启动子:	--
克隆方法:	多克隆位点，限制性内切酶
载体大小:	7032bp
5' 测序引物及序列:	GACTATCATATGCTTACCGT
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	嘌呤霉素
克隆菌株:	Stbl3 , E.coli
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	慢病毒

 

pLK0.1-puro载体质粒图谱和多克隆位点信息

 

pLK0.1-puro载体简介

 

pLK0.1-puro载体序列

下面是pLKO.1-puro载体无插入片段情况下的载体序列。LOCUS       pLKO.1 puro	7032 bp 	DNA  circular	SYNDEFINITION  pLKO.1 puroACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     source          1..7032                     /organism="pLKO.1 puro"                     /mol_type="other DNA"     CDS             289..1128                     /label="ORF frame 1"     CDS             complement(334..1179)                     /label="ORF frame 1"     gene            529..1128                     /label="puro (variant)"                     /gene="puro (variant)"     misc_feature    1239..1260                     /label="U3PPT"     misc_feature    1239..1254                     /label="cPPT"     misc_feature    1256..1308                     /label="delta_U3"     misc_feature    1309..1489                     /label="HIV-1_5_LTR"     misc_feature    1309..1489                     /label="truncHIV-1_3_LTR"     terminator      1564..1683                     /label="SV40_PA_terminator"     misc_feature    1652..1671                     /label="EBV_rev_primer"     rep_origin      1701..1799                     /label="SV40_origin"     misc_feature    1784..1803                     /label="SV40pro_F_primer"     promoter        complement(1878..1896)                     /label="T7_promoter"     promoter        complement(1906..1922)                     /label="M13_forward20_primer"     misc_feature    complement(1915..1937)                     /label="M13_pUC_fwd_primer"     misc_feature    1915..2063                     /label="lacZ_a"     rep_origin      2081..2387                     /label="f1_origin"     promoter        2580..2608                     /label="AmpR_promoter"     gene            2650..3510                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             2650..3510                     /label="ORF frame 1"     rep_origin      3665..4284                     /label="pBR322_origin"     promoter        4593..4622                     /label="lac_promoter"     misc_feature    4636..4658                     /label="M13_pUC_rev_primer"     promoter        4657..4675                     /label="M13_reverse_primer"     promoter        4692..4711                     /label="T3_promoter"     misc_feature    4965..5145                     /label="HIV-1_5_LTR"     misc_feature    4965..5145                     /label="truncHIV-1_3_LTR"     misc_feature    5256..5300                     /label="HIV-1_psi_pack"     CDS             5688..6545                     /label="ORF frame 3"     misc_feature    5810..6043                     /label="RRE"     promoter        6579..6811                     /label="hU6_promoter"     misc_feature    6741..6760                     /label="LKO1_5_primer"     misc_feature    6867..6882                     /label="cPPT"     CDS             complement(6948..530)                     /label="ORF frame 2"ORIGIN    1 ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg   61 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt  121 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct  181 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa  241 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc  301 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc  361 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc  421 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt  481 gaccgaatca ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac  541 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc  601 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag  661 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg  721 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg  781 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc  841 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc  901 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg  961 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 1021 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 1081 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac 1141 gacccgcagc gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca 1201 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa 1261 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg 1321 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 1381 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 1441 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca 1501 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg 1561 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 1621 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 1681 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc 1741 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 1801 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 1861 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt 1921 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 1981 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 2041 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 2101 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 2161 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 2221 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 2281 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 2341 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 2401 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 2461 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta 2521 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 2581 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 2641 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 2701 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 2761 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 2821 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 2881 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 2941 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 3001 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 3061 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 3121 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 3181 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 3241 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 3301 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 3361 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 3421 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 3481 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 3541 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 3601 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 3661 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 3721 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 3781 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag 3841 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 3901 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 3961 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 4021 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 4081 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 4141 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 4201 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 4261 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 4321 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 4381 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 4441 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 4501 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 4561 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 4621 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 4681 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt 4741 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct 4801 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg 4861 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc 4921 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag 4981 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 5041 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 5101 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 5161 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga 5221 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 5281 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 5341 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 5401 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 5461 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 5521 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 5581 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 5641 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 5701 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 5761 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 5821 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg 5881 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 5941 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 6001 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 6061 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 6121 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca 6181 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag 6241 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc 6301 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt 6361 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga 6421 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag 6481 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag 6541 actagcctcg agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt 6601 tgcatatacg atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa 6661 agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt 6721 taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt 6781 tcttggcttt atatatcttg tggaaaggac gaaacaccgg tgaattctcg acctcgagac 6841 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag 6901 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 6961 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc 7021 ggctcgaggg gg