pDC411

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pDC411

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pDC411

载体基本信息

出品公司:	Ybscience
载体名称:	pDC411
质粒类型:	腺病毒表达载体
高拷贝/低拷贝:	--
启动子:	--
克隆方法:	--
载体大小:	5574 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素（Ampicillin）
筛选标记:	--
备注:	克隆宿主菌：DB3.1
稳定性:	--
组成型:	组成型
病毒/非病毒:	腺病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pDC411       5574 bp dsDNA    CircularDEFINITION  Puc based shuttle plasmid  for Ad vector rescue via homologous recomb. Ng et al. Hum. Gene Ther. 11: xxx, 2000.COMMENT     This file is best viewed with a free Vector NTI Viewer:COMMENT     http://www.informaxinc.com/view/viewer.htmlCOMMENT     VNTREPLTYPE|Plasmid|COMMENT     VNTEXTCHREPL|Bacteria|FEATURES               Location/Qualifiers     misc_feature    515..2785                     /note="Main body of the Ad5 sequences present in this vector system with E1 deleted but with a normal packaging signal."                     /label=Ad\5\E1\deleted     CDS             600..1019                     /note="Protein IX open reading frame only, does NOT include promoter/ terminator etc."                     /label=pIX     rep_origin      3264..3564                     /label=ori     misc_feature    5115..453                     /label=Ad     CDS             complement(4014..4830)                     /label=amp     CDS             5484..1                     /label=ITR     CDS             complement(2..102)                     /label=ITRORIGIN        1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt        61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg       121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg       181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt       241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa       301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg       361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc       421 gggtcaaagt tggcgtttta ttattatagt cagntctaga gaattcaggc ctgctagcag       481 atctaagctt gagctcgtcg actcgaagat cgatctgggc gtggttaagg gtgggaaaga       541 atatataagg tgggggtctt atgtagtttt gtatctgttt tgcagcagcc gccgccgcca       601 tgagcaccaa ctcgtttgat ggaagcattg tgagctcata tttgacaacg cgcatgcccc       661 catgggccgg ggtgcgtcag aatgtgatgg gctccagcat tgatggtcgc cccgtcctgc       721 ccgcaaactc tactaccttg acctacgaga ccgtgtctgg aacgccgttg gagactgcag       781 cctccgccgc cgcttcagcc gctgcagcca ccgcccgcgg gattgtgact gactttgctt       841 tcctgagccc gcttgcaagc agtgcagctt cccgttcatc cgcccgcgat gacaagttga       901 cggctctttt ggcacaattg gattctttga cccgggaact taatgtcgtt tctcagcagc       961 tgttggatct gcgccagcag gtttctgccc tgaaggcttc ctcccctccc aatgcggttt      1021 aaaacataaa taaaaaacca gactctgttt ggatttggat caagcaagtg tcttgctgtc      1081 tttatttagg ggttttgcgc gcgcggtagg cccgggacca gcggtctcgg tcgttgaggg      1141 tcctgtgtat tttttccagg acgtggtaaa ggtgactctg gatgttcaga tacatgggca      1201 taagcccgtc tctggggtgg aggtagcacc actgcagagc ttcatgctgc ggggtggtgt      1261 tgtagatgat ccagtcgtag caggagcgct gggcgtggtg cctaaaaatg tctttcagta      1321 gcaagctgat tgccaggggc aggcccttgg tgtaagtgtt tacaaagcgg ttaagctggg      1381 atgggtgcat acgtggggat atgagatgca tcttggactg tatttttagg ttggctatgt      1441 tcccagccat atccctccgg ggattcatgt tgtgcagaac caccagcaca gtgtatccgg      1501 tgcacttggg aaatttgtca tgtagcttag aaggaaatgc gtggaagaac ttggagacgc      1561 ccttgtgacc tccaagattt tccatgcatt cgtccataat gatggcaatg ggcccacggg      1621 cggcggcctg ggcgaagata tttctgggat cactaacgtc atagttgtgt tccaggatga      1681 gatcgtcata ggccattttt acaaagcgcg ggcggagggt gccagactgc ggtataatgg      1741 ttccatccgg cccaggggcg tagttaccct cacagatttg catttcccac gctttgagtt      1801 cagatggggg gatcatgtct acctgcgggg cgatgaagaa aacggtttcc ggggtagggg      1861 agatcagctg ggaagaaagc aggttcctga gcagctgcga cttaccgcag ccggtgggcc      1921 cgtaaatcac acctattacc gggtgcaact ggtagttaag agagctgcag ctgccgtcat      1981 ccctgagcag gggggccact tcgttaagca tgtccctgac tcgcatgttt tccctgacca      2041 aatccgccag aaggcgctcg ccgcccagcg atagcagttc ttgcaaggaa gcaaagtttt      2101 tcaacggttt gagaccgtcc gccgtaggca tgcttttgag cgtttgacca agcagttcca      2161 ggcggtccca cagctcggtc acctgctcta cggcatctcg atccagcata tctcctcgtt      2221 tcgcgggttg gggcggcttt cgctgtacgg cagtagtcgg tgctcgtcca gacgggccag      2281 ggtcatgtct ttccacgggc gcagggtcct cgtcagcgta gtctgggtca cggtgaaggg      2341 gtgcgctccg ggctgcgcgc tggccagggt gcgcttgagg ctggtcctgc tggtgctgaa      2401 gcgctgccgg tcttcgccct gcgcgtcggc caggtagcat ttgaccatgg tgtcatagtc      2461 cagcccctcc gcggcgtggc ccttggcgcg cagcttgccc ttggaggagg cgccgcacga      2521 ggggcagtgc agacttttga gggcgtagag cttgggcgcg agaaataccg attccgggga      2581 gtaggcatcc gcgccgcagg ccccgcagac ggtctcgcat tccacgagcc aggtgagctc      2641 tggccgttcg gggtcaaaaa ccaggtttcc cccatgcttt ttgatgcgtt tcttacctct      2701 ggtttccatg agccggtgtc cacgctcggt gacgaaaagg ctgtccgtgt ccccgtatac      2761 agacttgaga ggcctgtcct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct      2821 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc      2881 aactcgtagg acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct      2941 ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc      3001 aagccttcgt cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg      3061 gcatggcggc cctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg      3121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag      3181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag      3241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc      3301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc      3361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc      3421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt      3481 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg      3541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat      3601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag      3661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt      3721 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc      3781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta      3841 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag      3901 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga      3961 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa      4021 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa      4081 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc      4141 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga      4201 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa      4261 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt      4321 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg      4381 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc      4441 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg      4501 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag      4561 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt      4621 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt      4681 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac      4741 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac      4801 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag      4861 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa      4921 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga      4981 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc      5041 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa      5101 ataggcgtat cactctaggc aaaatagcac cctcccgctc cagaacaaca tacagcgctt      5161 cacagcggca gcctaacagt cagccttacc agtaaaaaag aaaacctatt aaaaaaacac      5221 cactcgacac ggcaccagct caatcagtca cagtgtaaaa aagggccaag tgcagagcga      5281 gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc acaaaaaaca cccagaaaac      5341 cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc cacaacttcc tcaaatcgtc      5401 acttccgttt tcccacgtta cgtaacttcc cattttaaga aaactacaat tcccaacaca      5461 tacaagttac tccgccctaa aacctacgtc acccgccccg ttcccacgcc ccgcgccacg      5521 tcacaaactc caccccctca ttatcatatt ggcttcaatc caaaataagg tata