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保存条件 ：-20℃
保质期 ：6个月
英文名 ：pAAV-CA
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pAAV-CA载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pAAV-CA |
| 质粒类型: | 腺病毒载体； Cre-LoxP重组酶系统 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CAG (CMV enhancer/chicken β-actin promoter) |
| 载体大小: | 6060 bp |
| 5' 测序引物及序列: | XpAAVE-F (GCTAACCATGTTCATGCCTTC) |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 无 |
| 克隆菌株: | Stbl3 或 NEB Stble |
| 宿主细胞（系）: | 常规哺乳动物细胞系 |
| 备注: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 腺病毒 |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pAAV-CA 6060 bp DNA SYNDEFINITION pAAV-CAACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors.FEATURES Location/Qualifiers source 1..6060 /organism="pAAV-CA" /mol_type="other DNA" misc_feature 239..526 /label="CAG_enhancer" CDS 410..889 /label="ORF frame 2" /translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMG RGEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGE VRRQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD*" CDS complement(998..1501) /label="ORF frame 3" /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG TASPAPRPVRSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT KGPPGAL*" misc_feature 1836..1855 /label="pCAG_F_primer" /translation="MAAARRASTAAGAPGPPRPSPEPSPARGGPAPPGTPTCRHPPPG TASPAPRPVRSPAPEAGPCSATRGGGCRGGLQPDRRAHTPCSPPHAHTPHAAFVPLAA PPHRGAPPPAALPSRTRGAHKAPRRARSAHSRRAARAARGAPHAHTHARTPRAAPPRT KGPPGAL*" CDS 2145..2888 /label="ORF frame 3" /translation="MYAIRGYRMLELIDNQPLDYKICERLTGILNYVAPFTLCGYAAL MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVATW ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALLE RSTGGIPVTPPQCLSWPWKLPLQCPPALS*" misc_feature 2189..2776 /label="WPRE" /translation="MYAIRGYRMLELIDNQPLDYKICERLTGILNYVAPFTLCGYAAL MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTC QLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKSSSFPWLLAYVATW ILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHRYRALLE RSTGGIPVTPPQCLSWPWKLPLQCPPALS*" CDS 2290..2823 /label="ORF frame 1" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" terminator 2805..3283 /label="hGH_PA_terminator" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" rep_origin 3555..3861 /label="f1_origin" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" misc_feature 4129..4151 /label="pGEX_3_primer" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" promoter 4310..4338 /label="AmpR_promoter" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" gene 4380..5240 /label="Ampicillin" /gene="Ampicillin" /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF LGCSPMLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP PRIDTERCSRDLRVASL*" CDS 4380..5240 /label="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 5395..6014 /label="pBR322_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*"ORIGIN 1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtAAGCT GGGctgcagg aatctagtta 181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac 241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc 301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt 361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac 421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg 541 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa 601 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg 661 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg 721 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg 781 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc 841 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc 901 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa 961 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct 1021 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc 1081 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg 1141 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg 1201 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg 1261 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc 1321 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg 1381 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg 1441 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca 1501 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgTgc 1561 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggG cgaagcggtg 1621 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgcCgtcc 1681 ccttctccCt ctccagcctc ggggctgTcc gcGgggggac ggctgccttc gggggggacg 1741 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca 1801 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggttA ttgtgctgtc 1861 tcatcatttt ggcaaagaat tgatttgata cgtacggttg aattcaTTAC CTCGTATAGG 1921 ATACTTTATA CGAAGTTATG CAGAATGGTA GCTGGATTGT AAGTACTATT AGCAATATGA 1981 AACCTCTTAA TAACCTCGTA TAGCATACAT TATACGAAGT AATAgatcta agcttgatat 2041 cgtcGACATA ACTTCGTATA AAGTATCCTA TACGAGGTAA TTTGCCTTAA CCCAGAAATT 2101 ATCAGTACTA TTCTTTAGAA TGGTGCAAAG AATTACTTCG TATAATGTAT GCTATACGAG 2161 GTTATCGCAT Gctagagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat 2221 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc 2281 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct 2341 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca 2401 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt 2461 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg 2521 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga 2581 aatcatcgtc ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt 2641 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc 2701 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt 2761 gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg 2821 tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct 2881 tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat 2941 ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg 3001 cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc 3061 cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc 3121 atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt 3181 ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt 3241 gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac 3301 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg 3361 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc 3421 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat 3481 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg 3541 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 3601 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 3661 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 3721 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat 3781 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 3841 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag 3901 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 3961 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct 4021 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac 4081 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 4141 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac 4201 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 4261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 4321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 4381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 4441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 4501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 4561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 4621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 4681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 4741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 4801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 4861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 4921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 4981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 5041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 5101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 5161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 5221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 5281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 5341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 5401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 5461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 5521 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 5581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 5641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 5701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 5761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 5821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 5881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 5941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 6001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatg
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