pAAV-CaMKIIa-EGFP

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pAAV-CaMKIIa-EGFP

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pAAV-CaMKIIa-EGFP

载体基本信息

出品公司:	Ybscience
载体名称:	pAAV-CaMKIIa-EGFP
质粒类型:	腺病毒载体；荧光报告载体
高拷贝/低拷贝:	低拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CaMKIIa
载体大小:	6092 bp
5' 测序引物及序列:	ATGCTGACGAAGGCTCGCGA
3' 测序引物及序列:	GCATTAAAGCAGCGTATCCACATAGC
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	EGFP
克隆菌株:	Stbl3 或 NEB Stble
宿主细胞（系）:	神经细胞
备注:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	腺病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pAAV-CaMKIIa-EGFP	6092 bp 	DNA	SYNDEFINITION  pAAV-CaMKIIa-EGFPCOMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     ORIGDB|GenBankCOMMENT     VNTAUTHORNAME|biofeng.com|COMMENT     VNTNAME|pAAV-CaMKIIa-EGFP|       ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..6092                     /organism="pAAV-CaMKIIa-EGFP"                     /mol_type="other DNA"     CDS             1475..2194                     /label="ORF frame 2"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     gene            1478..2191                     /label="EGFP"                     /gene="EGFP"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    complement(1520..1541)                     /label="EGFP_N_primer"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    1658..1687                     /label="Y66 (EGFP)"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    2128..2149                     /label="EGFP_C_primer"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     misc_feature    2221..2808                     /label="WPRE"                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH                     MVLLEFVTAAGITLGMDELYK*"     CDS             2309..2920                     /label="ORF frame 2"                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVF                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK                     SSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS                     LWAASPHRYRALLERSTGGIPVTPPQCLSWPWKLPLQCPPALS*"     CDS             2322..2855                     /label="ORF frame 3"                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP                     PRIDTERCSRDLRVASL*"     terminator      2837..3315                     /label="hGH_PA_terminator"                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP                     PRIDTERCSRDLRVASL*"     rep_origin      3587..3893                     /label="f1_origin"                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP                     PRIDTERCSRDLRVASL*"     misc_feature    4161..4183                     /label="pGEX_3_primer"                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP                     PRIDTERCSRDLRVASL*"     promoter        4342..4370                     /label="AmpR_promoter"                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP                     PRIDTERCSRDLRVASL*"     gene            4412..5272                     /label="Ampicillin"                     /gene="Ampicillin"                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQP                     PLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPF                     LGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPP                     PRIDTERCSRDLRVASL*"     CDS             4412..5272                     /label="ORF frame 2"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     rep_origin      5427..6046                     /label="pBR322_origin"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc   61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca  121 actccatcac taggggttcc tgcggccgca cgcgtttaac attatggcct taggtcactt  181 catctccatg gggttcttct tctgattttc tagaaaatga gatgggggtg cagagagctt  241 cctcagtgac ctgcccaggg tcacatcaga aatgtcagag ctagaacttg aactcagatt  301 actaatctta aattccatgc cttgggggca tgcaagtacg atatacagaa ggagtgaact  361 cattagggca gatgaccaat gagtttagga aagaagagtc cagggcaggg tacatctaca  421 ccacccgccc agccctgggt gagtccagcc acgttcacct cattatagtt gcctctctcc  481 agtcctacct tgacgggaag cacaagcaga aactgggaca ggagccccag gagaccaaat  541 cttcatggtc cctctgggag gatgggtggg gagagctgtg gcagaggcct caggaggggc  601 cctgctgctc agtggtgaca gataggggtg agaaagcaga cagagtcatt ccgtcagcat  661 tctgggtctg tttggtactt cttctcacgc taaggtggcg gtgtgatatg cacaatggct  721 aaaaagcagg gagagctgga aagaaacaag gacagagaca gaggccaagt caaccagacc  781 aattcccaga ggaagcaaag aaaccattac agagactaca agggggaagg gaaggagaga  841 tgaattagct tcccctgtaa accttagaac ccagctgttg ccagggcaac ggggcaatac  901 ctgtctcttc agaggagatg aagttgccag ggtaactaca tcctgtcttt ctcaaggacc  961 atcccagaat gtggcaccca ctagccgtta ccatagcaac tgcctctttg ccccacttaa 1021 tcccatcccg tctgttaaaa gggccctata gttggaggtg ggggaggtag gaagagcgat 1081 gatcacttgt ggactaagtt tgttcgcatc cccttctcca accccctcag tacatcaccc 1141 tgggggaaca gggtccactt gctcctgggc ccacacagtc ctgcagtatt gtgtatataa 1201 ggccagggca aagaggagca ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt 1261 taccggggca acgggaacag ggcgtttcgg aggtggttgc catggggacc tggatgctga 1321 cgaaggctcg cgaggctgtg agcagccaca gtgccctgct cagaagcccc aagctcgtca 1381 gtcaagccgg ttctccgttt gcactcagga gcacgggcag gcgagtggcc cctagttctg 1441 ggggcagctc tagagcggta ccggatccgc caccatggtg agcaagggcg aggagctgtt 1501 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag 1561 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg 1621 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt 1681 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat 1741 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac 1801 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat 1861 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca 1921 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg 1981 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat 2041 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag 2101 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg 2161 gatcactctc ggcatggacg agctgtacaa gtaggaattc gatatcaagc ttatcgataa 2221 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc 2281 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat 2341 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg 2401 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg 2461 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat 2521 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt 2581 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc 2641 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa 2701 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg 2761 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgagc 2821 gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc tctcctggcc 2881 ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag ttgcatcatt 2941 ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt atggagcaag 3001 gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc aagctggagt 3061 gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc gattctcctg 3121 cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag ctaatttttg 3181 tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa ctcctaatct 3241 caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg aaccactgct 3301 cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg ccgcaggaac 3361 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc 3421 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc 3481 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt 3541 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc 3601 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc 3661 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa 3721 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact 3781 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt 3841 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa 3901 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg cctattggtt 3961 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac 4021 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg 4081 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 4141 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 4201 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat 4261 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat 4321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 4381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 4441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 4501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 4561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 4621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 4681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 4741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 4801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 4861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 4921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 4981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 5041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 5101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 5161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 5221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 5281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 5341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 6061 cctggccttt tgctggcctt ttgctcacat gt