pVL1393

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pVL1393

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pVL1393

载体基本信息

出品公司:	Ybscience
载体名称:	pVL1393
质粒类型:	昆虫表达载体；杆状病毒载体
克隆方法:	限制性内切酶，多克隆位点
启动子:	Polyhedrin
载体大小:	9632 bp
5' 测序引物及序列:	Polyhedrin Forward：5' d(AAA TGA TAA CCA TCT CGC) 3'
3' 测序引物及序列:	Polyhedrin Reverse：5' d(GTC CAA GTT TCC CTG) 3'
载体标签:	无标签
载体抗性:	氨苄青霉素
真核筛选标记:	无
克隆菌株:	推荐E.coli TOP10
宿主细胞（系）:	Sf9、Sf21、High Five细胞系
备注:	相关载体有pAC360, pVL1392
瞬表达/稳表达:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	杆状病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

pVL1393载体和pVL1392载体简介pVL1392 and pVL1393 are 9.8 kb vectors designed for use as baculovirus transfer vectors. These transfer vectors contain recombination sequences which are homologous to sequences in the baculovirus genome.Upon cotransfection, these sequences undergo homologous recombination resulting in a recombinant viral genome packaged in infectious viral particles.These vectors are nonfusion vectors, which provide the AcMNPV polyhedrin enhancer-promoter sequences to drive high expression, and a multiple cloning site, conveniently constructed in opposite orientations, for simple subcloning. Translation initiation sequences must be provided by the inserted cDNA.载体特征• Multiple cloning site polylinker, positioned in opposite orientations• Recombination sequences for insertion into the baculovirus genome• Polyhedrin enhancer-promoter sequences for high expression of recombinant proteinFeatures for growth and maintenance in E. coli:• Ampicillin gene for selection• pMB1 origin of replication from pUC19 for high copy number growth载体复制The TOP10 cells are provided as a stab.TOP10 is provided for growth and maintenance of this plasmid. TOP10 is a recombination-negative strain designed for stable replication of high copy number plasmids. This strain is provided as a convenience for those who do not have access to other E. coli strains. Many E. coli strains are suitable for the growth of this vector including DH5α, DH10, and INVαF'. In general, it is best to grow vectors containing inserts in E. coli strains which are recombination-deficient (recA-).Genotype of TOP10F-, mcrA ∆(mrr-hsdRMS-mcrBC) Φ80lacZ∆M15 ∆lacX74 deoR recA1 araD139 ∆(ara-leu)7697 galU galK rpsL endA1 nupG 

 

载体序列

LOCUS       pVL1393	9632 bp 	DNA	SYNDEFINITION  pVL1393ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..9632                     /organism="pVL1393"                     /mol_type="other DNA"     CDS             76..582                     /label="ORF frame 1"     CDS             complement(614..1600)                     /label="ORF frame 2"     CDS             complement(1868..2323)                     /label="ORF frame 2"     CDS             2662..3294                     /label="ORF frame 1"     misc_feature    2844..3990                     /label="Ac_5_flank"     CDS             complement(3332..3937)                     /label="ORF frame 2"     promoter        3991..4091                     /label="PH_promoter"     misc_feature    4017..4034                     /label="polyhedrin_fwd_primer"     CDS             4250..4741                     /label="ORF frame 2"     misc_feature    complement(4288..4302)                     /label="polyhedrinn_rev_primer"     misc_feature    4640..4741                     /label="3_AcPH"     misc_feature    complement(4657..4674)                     /label="Baculovirs_rev_primer"     misc_feature    4755..6180                     /label="Ac_3_flank"     CDS             complement(4771..6024)                     /label="ORF frame 3"     misc_feature    complement(4853..5651)                     /label="r_ORF1629"     CDS             6397..6987                     /label="ORF frame 1"     promoter        complement(7009..7027)                     /label="M13_reverse_primer"     misc_feature    complement(7026..7048)                     /label="M13_pUC_rev_primer"     promoter        complement(7062..7091)                     /label="lac_promoter"     gene            complement(8174..9034)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(8174..9034)                     /label="ORF frame 2"     promoter        complement(9076..9104)                     /label="AmpR_promoter"     misc_feature    complement(9263..9285)                     /label="pGEX_3_primer"     misc_feature    complement(9484..9627)                     /label="lacZ_a"     misc_feature    9598..9620                     /label="M13_pUC_fwd_primer"     promoter        9613..9629                     /label="M13_forward20_primer"ORIGIN    1 aagctttact cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt   61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt  121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac  181 gtgactagcg aagaagatgt gtggaccgca gaacagatag taaaacaaaa ccctagtatt  241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg  301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata  361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg  421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg  481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac  541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc  601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta  661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag  721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt  781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca  841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat  901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt  961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg 1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa 1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg 1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt 1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa 1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc 1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga 1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat 1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt 1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat 1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat 1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa 1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc 1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag 1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt 1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc 1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg 1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt 2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg 2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat 2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca 2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca 2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc 2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac 2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg 2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca 2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt 2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc 2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt 2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat 2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc 2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc 2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa 2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg 3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta 3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag 3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta 3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag 3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata 3301 aaacaattat aaatgctaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa 3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa 3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac 3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa 3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt 3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc 3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta 3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt 3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt 3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt 3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat 3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat 4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa 4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccgggta 4141 ccttctagaa ttccggagcg gccgctgcag atctgatcct ttcctgggac ccggcaagaa 4201 ccaaaaactc actctcttca aggaaatccg taatgttaaa cccgacacga tgaagcttgt 4261 cgttggatgg aaaggaaaag agttctacag ggaaacttgg acccgcttca tggaagacag 4321 cttccccatt gttaacgacc aagaagtgat ggatgttttc cttgttgtca acatgcgtcc 4381 cactagaccc aaccgttgtt acaaattcct ggcccaacac gctctgcgtt gcgaccccga 4441 ctatgtacct catgacgtga ttaggatcgt cgagccttca tgggtgggca gcaacaacga 4501 gtaccgcatc agcctggcta agaagggcgg cggctgccca ataatgaacc ttcactctga 4561 gtacaccaac tcgttcgaac agttcatcga tcgtgtcatc tgggagaact tctacaagcc 4621 catcgtttac atcggtaccg actctgctga agaggaggaa attctccttg aagtttccct 4681 ggtgttcaaa gtaaaggagt ttgcaccaga cgcacctctg ttcactggtc cggcgtatta 4741 aaacacgata cattgttatt agtacattta ttaagcgcta gattctgtgc gttgttgatt 4801 tacagacaat tgttgtacgt attttaataa ttcattaaat ttataatctt tagggtggta 4861 tgttagagcg aaaatcaaat gattttcagc gtctttatat ctgaatttaa atattaaatc 4921 ctcaatagat ttgtaaaata ggtttcgatt agtttcaaac aagggttgtt tttccgaacc 4981 gatggctgga ctatctaatg gattttcgct caacgccaca aaacttgcca aatcttgtag 5041 cagcaatcta gctttgtcga tattcgtttg tgttttgttt tgtaataaag gttcgacgtc 5101 gttcaaaata ttatgcgctt ttgtatttct ttcatcactg tcgttagtgt acaattgact 5161 cgacgtaaac acgttaaata aagcttggac atatttaaca tcgggcgtgt tagctttatt 5221 aggccgatta tcgtcgtcgt cccaaccctc gtcgttagaa gttgcttccg aagacgattt 5281 tgccatagcc acacgacgcc tattaattgt gtcggctaac acgtccgcga tcaaatttgt 5341 agttgagctt tttggaatta tttctgattg cgggcgtttt tgggcgggtt tcaatctaac 5401 tgtgcccgat tttaattcag acaacacgtt agaaagcgat ggtgcaggcg gtggtaacat 5461 ttcagacggc aaatctacta atggcggcgg tggtggagct gatgataaat ctaccatcgg 5521 tggaggcgca ggcggggctg gcggcggagg cggaggcgga ggtggtggcg gtgatgcaga 5581 cggcggttta ggctcaaatg tctctttagg caacacagtc ggcacctcaa ctattgtact 5641 ggtttcgggc gccgtttttg gtttgaccgg tctgagacga gtgcgatttt tttcgtttct 5701 aatagcttcc aacaattgtt gtctgtcgtc taaaggtgca gcgggttgag gttccgtcgg 5761 cattggtgga gcgggcggca attcagacat cgatggtggt ggtggtggtg gaggcgctgg 5821 aatgttaggc acgggagaag gtggtggcgg cggtgccgcc ggtataattt gttctggttt 5881 agtttgttcg cgcacgattg tgggcaccgg cgcaggcgcc gctggctgca caacggaagg 5941 tcgtctgctt cgaggcagcg cttggggtgg tggcaattca atattataat tggaatacaa 6001 atcgtaaaaa tctgctataa gcattgtaat ttcgctatcg tttaccgtgc cgatatttaa 6061 caaccgctca atgtaagcaa ttgtattgta aagagattgt ctcaagctcg ccgcacgccg 6121 ataacaagcc ttttcatttt tactacagca ttgtagtggc gagacacttc gctgtcgtcg 6181 acgtacatgt atgctttgtt gtcaaaaacg tcgttggcaa gctttaaaat atttaaaaga 6241 acatctctgt tcagcaccac tgtgttgtcg taaatgttgt ttttgataat ttgcgcttcc 6301 gcagtatcga cacgttcaaa aaattgatgc gcatcaattt tgttgttcct attattgaat 6361 aaataagatt gtacagattc atatctacga ttcgtcatgg ccaccacaaa tgctacgctg 6421 caaacgctgg tacaatttta cgaaaactgc aaaaacgtca aaactcggta taaaataatc 6481 aacgggcgct ttggcaaaat atctatttta tcgcacaagc ccactagcaa attgtatttg 6541 cagaaaacaa tttcggcgca caattttaac gctgacgaaa taaaagttca ccagttaatg 6601 agcgaccacc caaattttat aaaaatctat tttaatcacg gttccatcaa caaccaagtg 6661 atcgtgatgg actacattga ctgtcccgat ttatttgaaa cactacaaat taaaggcgag 6721 ctttcgtacc aacttgttag caatattatt agacagctgt gtgaagcgct caacgatttg 6781 cacaagcaca atttcataca caacgacata aaactcgaaa atgtcttata tttcgaagca 6841 cttgatcgcg tgtatgtttg cgattacgga ttgtgcaaac acgaaaactc acttagcgtg 6901 cacgacggca cgttggagta ttttagtccg gaaaaaattc gacacacaac tatgcacgtt 6961 tcgtttgact ggtacgcggc gtgttaacat acaagttgct aacgtaatca tggtcatagc 7021 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 7081 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 7141 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 7201 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 7261 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 7321 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 7381 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 7441 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 7501 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 7561 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 7621 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 7681 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 7741 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 7801 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag 7861 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 7921 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 7981 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 8041 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 8101 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 8161 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 8221 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 8281 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 8341 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 8401 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 8461 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 8521 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 8581 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 8641 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 8701 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 8761 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 8821 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 8881 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 8941 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 9001 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 9061 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 9121 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca 9181 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc 9241 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt 9301 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg 9361 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata 9421 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc 9481 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc 9541 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc 9601 agtcacgacg ttgtaaaacg acggccagtg cc