pMETαA

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pMETαA

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pMETαA

pMETalpha A载体基本信息

出品公司:	Ybscience
载体名称:	pMETalpha A, pMETα A, pMETalphaA, pMETαA
质粒类型:	毕赤酵母蛋白表达载体（methanoloica酵母）
表达水平:	高拷贝
诱导方法:	甲醇诱导
启动子:	AUG1
克隆方法:	多克隆位点，限制性内切酶
载体大小:	8043 bp
5' 测序引物及序列:	AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´;  alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´
3' 测序引物及序列:	AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
载体标签:	C-V5， C-His
载体抗性:	氨苄
筛选标记:	ADE2
备注:	利用甲醇诱导蛋白在methanoloica酵母中表达。
稳定性:	稳定 Stable
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

pMETalpha A载体质粒图谱和多克隆位点信息

pMETalpha A载体简介

 

pMETalpha A载体序列

LOCUS       pMET\alpha\A            8043 bp    DNA     circular      1-AUG-2013DEFINITION  pMETα A.SOURCE        ORGANISM  other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     ORIGDB|GenBankCOMMENT     VNTNAME|pMET alpha A|COMMENT     VNTOAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     misc_feature    1106..1130                     /vntifkey="21"                     /label=AUG1_fwd_primer     misc_feature    complement(1662..1683)                     /vntifkey="21"                     /label=AUG1_rev_primer     CDS             3085..3660                     /vntifkey="4"                     /label=ORF\frame\3     CDS             3666..4799                     /vntifkey="4"                     /label=ORF\frame\2     CDS             4963..5961                     /vntifkey="4"                     /label=ORF\frame\3     gene            complement(6774..7634)                     /gene="Ampicillin"                     /vntifkey="60"     CDS             complement(6774..7634)                     /vntifkey="4"                     /label=ORF\frame\2     promoter        complement(7676..7704)                     /vntifkey="30"                     /label=AmpR_promoter     sig_peptide     1197..1463                     /vntifkey="94"                     /label=Alpha\Factor\Secretion\SignalBASE COUNT     2360 a      1741 c      1568 g      2374 t ORIGIN        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag        61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt       121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac       181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca       241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac       301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa       361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc       421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa       481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg       541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc       601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa       661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct       721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct       781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg       841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa       901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat       961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct      1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt      1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac      1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga      1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag      1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact      1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg      1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc      1441 tcgagaaaag agaggctgaa gctgaattca cgtggcccag ccggccgtgg atccacgcgt      1501 cgtcgacccg cggcggccgc cagcttccta ggggtaagcc tatccctaac cctctcctcg      1561 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgaactagt atacaattct      1621 agggctgcct gtttggatat ttttataatt tttgagagtt tgccaactaa tgtttttctc      1681 ttctatgata tttatcatgt agttggatat ctttttttct ttgaaaagtt tttatttagt      1741 tctttctctc ttttttataa tgttttcagt ttctttcctt tatattgaaa atacgatgtt      1801 ctttgtcttt cttttttaaa tcctcaatta taatgtgtac cgtttgatca acttgtaaaa      1861 ttgtttgtag tcctgtaatc ttaattgacg attggttgat agtttacccc agtatattgt      1921 gcggctgaga tattacccga ttcggaagat tgattttttc aacagacata aacccatagg      1981 agatttagta cagcgacata tgctaattgc ctgataatgc tcactaacat tttctccttt      2041 taatctggta tatgcataca ggtatgttga tatggccttg ttgatcaaag ctacgaaagt      2101 gtagactatt tcccaaacag cccccaacca ccacctcaca cgcgcacact gcgaagtgaa      2161 agcgctccct tattttcttc tccgctggtt caaaacaaaa aagaatctaa tctgtagaca      2221 cacgccccgt aaaacccaat cttcatcatc gtaattttca gtgacggacg atcgatcaac      2281 catatagttt gttagtgact gagcttcagt agttgacctc ttgaagaaat aacgtcttga      2341 acaacatact ttatacctac caacattcgc catcaacaac agtgtgacac tgacataact      2401 ttacatccat tcatcgacaa aaaaatgtct ctagttcgga ttcatgctta tgggttagct      2461 atttcgccca atgtgtccat ctgacattac tattttgcat tttaatttaa ttagaacttg      2521 actagcgcac taccagcata tcatctcatt tccgtaaata ccaaatgtat tatatattga      2581 aagcttttga ccaggttatt ataaaagaaa cttcatgctc gaaaaagatc atttcgaaaa      2641 gttgcctagt ttcatgaaat tttaaagcag tttatataaa ttttaccttt tgatgcggaa      2701 ttgacttttt cttgaataat acataacttt tcttaaaaga atcaaagaca gataaaattt      2761 aagagatatt aaatattagt gagaagccga gaattttgta acaccaacat aacactgaca      2821 tctttaacaa cttttaatta tgatacattt cttacgtcat gattgattat tacagctatg      2881 ctgacaaatg actcttgttg catggctacg aaccgggtaa tactaagtga ttgactcttg      2941 ctgacctttt attaagaact aaatggacaa tattatggag catttcatgt ataaattggt      3001 gcgtaaaatc gttggatctc tcttctaagt acatcctact ataacaatca agaaaaacaa      3061 gaaaatcgga caaaacaatc aagtatggat tctagaacag ttggtatatt aggaggggga      3121 caattgggac gtatgattgt tgaggcagca aacaggctca acattaagac ggtaatacta      3181 gatgctgaaa attctcctgc caaacaaata agcaactcca atgaccacgt taatggctcc      3241 ttttccaatc ctcttgatat cgaaaaacta gctgaaaaat gtgatgtgct aacgattgag      3301 attgagcatg ttgatgttcc tacactaaag aatcttcaag taaaacatcc caaattaaaa      3361 atttaccctt ctccagaaac aatcagattg atacaagaca aatatattca aaaagagcat      3421 ttaatcaaaa atggtatagc agttacccaa agtgttcctg tggaacaagc cagtgagacg      3481 tccctattga atgttggaag agatttgggt tttccattcg tcttgaagtc gaggactttg      3541 gcatacgatg gaagaggtaa cttcgttgta aagaataagg aaatgattcc ggaagctttg      3601 gaagtactga aggatcgtcc tttgtcgccg aaaaatgggc accatttact aaagaattag      3661 cagtcatgat tgtgagatct gttaacggtt tagtgttttc ttacccaatt gtagagacta      3721 tccacaagga caatatttgt gacttatgtt atgcgcctgc tagagttccg gactccgttc      3781 aacttaaggc gaagttgttg gcagaaaatg caatcaaatc ttttcccggt tgtggtatat      3841 ttggtgtgga aatgttctat ttagaaacag gggaattgct tattaacgaa attgccccaa      3901 ggcctcacaa ctctggacat tataccattg atgcttgcgt cacttctcaa tttgaagctc      3961 atttgagatc aatattggat ttgccaatgc caaagaattt cacatctttc tccaccatta      4021 caacgaacgc cattatgcta aatgttcttg gagacaaaca tacaaaagat aaagagctag      4081 aaacttgcga aagagcattg gcgactccag gttcctcagt gtacttatat ggaaaagagt      4141 ctagacctaa cagaaaagta ggtcacataa atattattgc ctccagtatg gcggaatgtg      4201 aacaaaggct gaactacatt acaggtagaa ctgatattcc aatcaaaatc tctgtcgctc      4261 aaaagttgga cttggaagca atggtcaaac cattggttgg aatcatcatg ggatcagact      4321 ctgacttgcc ggtaatgtct gccgcatgtg cggttttaaa agattttggc gttccatttg      4381 aagtgacaat agtctctgct catagaactc cacataggat gtcagcatat gctatttccg      4441 caagcaagcg tggaattaaa acaattatcg ctggagctgg tggggctgct cacttgccag      4501 gtatggtggc tgcaatgaca ccacttcctg tcatcggtgt gcccgtaaaa ggttcttgtc      4561 tagatggagt agattcttta cattcaattg tgcaaatgcc tagaggtgtt ccagtagcta      4621 ccgtcgctat taataatagt acgaacgctg cgctgttggc tgtcagactg cttggcgctt      4681 atgattcaag ttatacaacg aaaatggaac agtttttatt aaagcaagaa gaagaagttc      4741 ttgtcaaagc acaaaagtta gaaactgtcg gttacgaagc ttatctagaa aacaagtaat      4801 atataagttt attgatatac ttgtacagca aataattata aaatgatata cctatttttt      4861 aggctttgtt atgattacat caaatgtgga cttcatacat agaaatcaac gcttacaggt      4921 gtcctttttt aagaatttca tacataagat ctactagaat caatgttaaa cctgttatca      4981 ttagaacagc catctccaga aggttctatc caagtgaaaa ctttggtctt caagccaaag      5041 actgctaaaa cacaaacccc agtcccaatt attgttgttg ctgaaacttc tacttctact      5101 ccgggtggtg ttatagccaa agcttcgggc actaaagagc caagattagc aaacgatgaa      5161 ttgaccaacc aattctttgg ttctcctgct aaagagtttt ctattgccag cttatcaaac      5221 gaacacaaag acggtaaagt caagatcatt attgatgctg ggatcaaaga tgatgactcg      5281 actaattacg ttatttccaa cactgttgct aagttagttt taaccggtaa gcaattatta      5341 ggtttcttat ccaactacga accaaccatc gttgatttta ctgctgaagc tgcaaccgct      5401 gccccaactc cagttgttaa gaaagaacct gctgcatctg gtaagcttga tgatgccaaa      5461 ttaattggtg ttactgctga caaggaaaaa gatttctcca actggtactc ccaaattcta      5521 atgaagggtg aaatgttaga ctactacgat gtctctggtt gttacattat gagaccagct      5581 tcttacacaa tttgggaaaa gattcaatct tggttcgatt cgaagattaa agaagatggt      5641 gttactaatg cttatttccc aatgtttgtc tcatccaagg ttttagaaaa ggaaaaggac      5701 cacatcgatg gtttcgctcc tgaagttgct tgggttacca gagctggtaa atctgaatta      5761 gaagaaccaa ttgctattag accaacttct gaaactgtta tgggtaccct gcaggggccg      5821 gccttaatta aggcgcgccc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg      5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat      5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc      6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc      6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga      6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt      6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg      6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc      6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg      6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc      6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg      6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc      6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct      6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt      6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa      6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa      6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc      6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct      6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca      6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt      7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt      7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc      7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc      7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt      7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact      7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc      7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt      7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg      7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct      7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa      7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt      7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc      7741 acatttcccc gaaaagtgcc acctgtatgc ggtgtgaaat accgcacaga tgcgtaagga      7801 gaaaataccg catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt      7861 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc      7921 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt      7981 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcaagggc gatggcccac      8041 tac