pMETαB

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pMETαB

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pMETαB

pMETalpha B载体基本信息

出品公司:	Ybscience
载体名称:	pMETalpha B, pMETα B, pMETalphaB, pMETαB
质粒类型:	毕赤酵母蛋白表达载体（methanoloica酵母）
表达水平:	高拷贝
诱导方法:	甲醇诱导
启动子:	AUG1
克隆方法:	多克隆位点，限制性内切酶
载体大小:	8048 bp
5' 测序引物及序列:	AUG1-F: 5´-CAATTTACATCTTTATTTATTAACG-3´;  alpha-Factor-F: 5´-TACTATTGCCAGCATTGCTGC-3´
3' 测序引物及序列:	AUG1-R: 5´-GAAGAGAAAAACATTAGTTGGC-3´
载体标签:	C-V5， C-His，N-alpha分泌因子
载体抗性:	氨苄
筛选标记:	ADE2
备注:	含有alpha factor酵母分泌信号肽，利用甲醇诱导蛋白在methanoloica酵母中分泌表达蛋白。
稳定性:	稳定 Stable
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

pMETalpha B载体质粒图谱和多克隆位点信息

pMETalpha B载体简介

 

pMETalpha B载体序列

LOCUS       pMET\alpha\B            8048 bp    DNA     circular      1-AUG-2013DEFINITION  pMET alpha B.SOURCE        ORGANISM  other sequences; artificial sequences; vectors.COMMENT     http://www.biofeng.com/COMMENT     ORIGDB|GenBankCOMMENT     VNTNAME|pMET alpha B|COMMENT     VNTOAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     misc_feature    1106..1130                     /vntifkey="21"                     /label=AUG1_fwd_primer     misc_feature    complement(1667..1688)                     /vntifkey="21"                     /label=AUG1_rev_primer     CDS             3090..3665                     /vntifkey="4"                     /label=ORF\frame\1     CDS             3671..4804                     /vntifkey="4"                     /label=ORF\frame\3     CDS             4968..5966                     /vntifkey="4"                     /label=ORF\frame\1     gene            complement(6779..7639)                     /gene="Ampicillin"                     /vntifkey="60"     CDS             complement(6779..7639)                     /vntifkey="4"                     /label=ORF\frame\2     promoter        complement(7681..7709)                     /vntifkey="30"                     /label=AmpR_promoter     sig_peptide     1197..1463                     /vntifkey="94"                     /label=Alpha\factor\secretion\signalBASE COUNT     2362 a      1741 c      1569 g      2376 t ORIGIN        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag        61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt       121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac       181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca       241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac       301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa       361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc       421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa       481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg       541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc       601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa       661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct       721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct       781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg       841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa       901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat       961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct      1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt      1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac      1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcaaaatga      1201 gatttccttc aatttttact gcagttttat tcgcagcatc ctccgcatta gctgctccag      1261 tcaacactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact      1321 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg      1381 ggttattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc      1441 tcgagaaaag agaggctgaa gctgcaggaa ttcacgtggc ccagccggcc gtggatccac      1501 gcgtcgtcga cccgcggcgg ccgccagctt actagtaggt aagcctatcc ctaaccctct      1561 cctcggtctc gattctacgc gtaccggtca tcatcaccat caccattgat ctagtataca      1621 attctagggc tgcctgtttg gatattttta taatttttga gagtttgcca actaatgttt      1681 ttctcttcta tgatatttat catgtagttg gatatctttt tttctttgaa aagtttttat      1741 ttagttcttt ctctcttttt tataatgttt tcagtttctt tcctttatat tgaaaatacg      1801 atgttctttg tctttctttt ttaaatcctc aattataatg tgtaccgttt gatcaacttg      1861 taaaattgtt tgtagtcctg taatcttaat tgacgattgg ttgatagttt accccagtat      1921 attgtgcggc tgagatatta cccgattcgg aagattgatt ttttcaacag acataaaccc      1981 ataggagatt tagtacagcg acatatgcta attgcctgat aatgctcact aacattttct      2041 ccttttaatc tggtatatgc atacaggtat gttgatatgg ccttgttgat caaagctacg      2101 aaagtgtaga ctatttccca aacagccccc aaccaccacc tcacacgcgc acactgcgaa      2161 gtgaaagcgc tcccttattt tcttctccgc tggttcaaaa caaaaaagaa tctaatctgt      2221 agacacacgc cccgtaaaac ccaatcttca tcatcgtaat tttcagtgac ggacgatcga      2281 tcaaccatat agtttgttag tgactgagct tcagtagttg acctcttgaa gaaataacgt      2341 cttgaacaac atactttata cctaccaaca ttcgccatca acaacagtgt gacactgaca      2401 taactttaca tccattcatc gacaaaaaaa tgtctctagt tcggattcat gcttatgggt      2461 tagctatttc gcccaatgtg tccatctgac attactattt tgcattttaa tttaattaga      2521 acttgactag cgcactacca gcatatcatc tcatttccgt aaataccaaa tgtattatat      2581 attgaaagct tttgaccagg ttattataaa agaaacttca tgctcgaaaa agatcatttc      2641 gaaaagttgc ctagtttcat gaaattttaa agcagtttat ataaatttta ccttttgatg      2701 cggaattgac tttttcttga ataatacata acttttctta aaagaatcaa agacagataa      2761 aatttaagag atattaaata ttagtgagaa gccgagaatt ttgtaacacc aacataacac      2821 tgacatcttt aacaactttt aattatgata catttcttac gtcatgattg attattacag      2881 ctatgctgac aaatgactct tgttgcatgg ctacgaaccg ggtaatacta agtgattgac      2941 tcttgctgac cttttattaa gaactaaatg gacaatatta tggagcattt catgtataaa      3001 ttggtgcgta aaatcgttgg atctctcttc taagtacatc ctactataac aatcaagaaa      3061 aacaagaaaa tcggacaaaa caatcaagta tggattctag aacagttggt atattaggag      3121 ggggacaatt gggacgtatg attgttgagg cagcaaacag gctcaacatt aagacggtaa      3181 tactagatgc tgaaaattct cctgccaaac aaataagcaa ctccaatgac cacgttaatg      3241 gctccttttc caatcctctt gatatcgaaa aactagctga aaaatgtgat gtgctaacga      3301 ttgagattga gcatgttgat gttcctacac taaagaatct tcaagtaaaa catcccaaat      3361 taaaaattta cccttctcca gaaacaatca gattgataca agacaaatat attcaaaaag      3421 agcatttaat caaaaatggt atagcagtta cccaaagtgt tcctgtggaa caagccagtg      3481 agacgtccct attgaatgtt ggaagagatt tgggttttcc attcgtcttg aagtcgagga      3541 ctttggcata cgatggaaga ggtaacttcg ttgtaaagaa taaggaaatg attccggaag      3601 ctttggaagt actgaaggat cgtcctttgt cgccgaaaaa tgggcaccat ttactaaaga      3661 attagcagtc atgattgtga gatctgttaa cggtttagtg ttttcttacc caattgtaga      3721 gactatccac aaggacaata tttgtgactt atgttatgcg cctgctagag ttccggactc      3781 cgttcaactt aaggcgaagt tgttggcaga aaatgcaatc aaatcttttc ccggttgtgg      3841 tatatttggt gtggaaatgt tctatttaga aacaggggaa ttgcttatta acgaaattgc      3901 cccaaggcct cacaactctg gacattatac cattgatgct tgcgtcactt ctcaatttga      3961 agctcatttg agatcaatat tggatttgcc aatgccaaag aatttcacat ctttctccac      4021 cattacaacg aacgccatta tgctaaatgt tcttggagac aaacatacaa aagataaaga      4081 gctagaaact tgcgaaagag cattggcgac tccaggttcc tcagtgtact tatatggaaa      4141 agagtctaga cctaacagaa aagtaggtca cataaatatt attgcctcca gtatggcgga      4201 atgtgaacaa aggctgaact acattacagg tagaactgat attccaatca aaatctctgt      4261 cgctcaaaag ttggacttgg aagcaatggt caaaccattg gttggaatca tcatgggatc      4321 agactctgac ttgccggtaa tgtctgccgc atgtgcggtt ttaaaagatt ttggcgttcc      4381 atttgaagtg acaatagtct ctgctcatag aactccacat aggatgtcag catatgctat      4441 ttccgcaagc aagcgtggaa ttaaaacaat tatcgctgga gctggtgggg ctgctcactt      4501 gccaggtatg gtggctgcaa tgacaccact tcctgtcatc ggtgtgcccg taaaaggttc      4561 ttgtctagat ggagtagatt ctttacattc aattgtgcaa atgcctagag gtgttccagt      4621 agctaccgtc gctattaata atagtacgaa cgctgcgctg ttggctgtca gactgcttgg      4681 cgcttatgat tcaagttata caacgaaaat ggaacagttt ttattaaagc aagaagaaga      4741 agttcttgtc aaagcacaaa agttagaaac tgtcggttac gaagcttatc tagaaaacaa      4801 gtaatatata agtttattga tatacttgta cagcaaataa ttataaaatg atatacctat      4861 tttttaggct ttgttatgat tacatcaaat gtggacttca tacatagaaa tcaacgctta      4921 caggtgtcct tttttaagaa tttcatacat aagatctact agaatcaatg ttaaacctgt      4981 tatcattaga acagccatct ccagaaggtt ctatccaagt gaaaactttg gtcttcaagc      5041 caaagactgc taaaacacaa accccagtcc caattattgt tgttgctgaa acttctactt      5101 ctactccggg tggtgttata gccaaagctt cgggcactaa agagccaaga ttagcaaacg      5161 atgaattgac caaccaattc tttggttctc ctgctaaaga gttttctatt gccagcttat      5221 caaacgaaca caaagacggt aaagtcaaga tcattattga tgctgggatc aaagatgatg      5281 actcgactaa ttacgttatt tccaacactg ttgctaagtt agttttaacc ggtaagcaat      5341 tattaggttt cttatccaac tacgaaccaa ccatcgttga ttttactgct gaagctgcaa      5401 ccgctgcccc aactccagtt gttaagaaag aacctgctgc atctggtaag cttgatgatg      5461 ccaaattaat tggtgttact gctgacaagg aaaaagattt ctccaactgg tactcccaaa      5521 ttctaatgaa gggtgaaatg ttagactact acgatgtctc tggttgttac attatgagac      5581 cagcttctta cacaatttgg gaaaagattc aatcttggtt cgattcgaag attaaagaag      5641 atggtgttac taatgcttat ttcccaatgt ttgtctcatc caaggtttta gaaaaggaaa      5701 aggaccacat cgatggtttc gctcctgaag ttgcttgggt taccagagct ggtaaatctg      5761 aattagaaga accaattgct attagaccaa cttctgaaac tgttatgggt accctgcagg      5821 ggccggcctt aattaaggcg cgcccttcct cgctcactga ctcgctgcgc tcggtcgttc      5881 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag      5941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa      6001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc      6061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc      6121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg      6181 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt      6241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc      6301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc      6361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag      6421 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg      6481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa      6541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag      6601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact      6661 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa      6721 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt      6781 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag      6841 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca      6901 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc      6961 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt      7021 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg      7081 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca      7141 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg      7201 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca      7261 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg      7321 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct      7381 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca      7441 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca      7501 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg      7561 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac      7621 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt      7681 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc      7741 cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt      7801 aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa attcgcgtta      7861 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat      7921 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca      7981 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca agggcgatgg      8041 cccactac