pYES2-NTC

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pYES2-NTC

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pYES2-NTC

载体基本信息

出品公司:	Ybscience
载体名称:	pYES2-NTC, pYES2/NT C
质粒类型:	酿酒酵母蛋白表达载体
表达水平:	高拷贝
诱导方法:	半乳糖
启动子:	GAL1
克隆方法:	多克隆位点，限制性内切酶
载体大小:	6037 bp
5' 测序引物及序列:	T7: TAATACGACTCACTATAGGG
3' 测序引物及序列:	CYC1 Terminator: GTGACATAACTAATTACATGATG
载体标签:	N-His, N-Xpress, C-His, C-V5
载体抗性:	氨苄
筛选标记:	URA3
备注:	利用半乳糖诱导蛋白在酿酒酵母中表达。
稳定性:	稳定 Stable
组成型/诱导型:	诱导型
病毒/非病毒:	非病毒

载体质粒图谱和多克隆位点信息

载体简介

pYES2 NTC的是一个6.0 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。

该载体包含以下元素：

1. 酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达

2.多克隆位点可以使用的很多限制酶切位点，便于基因插入。

3.CYC1终止子能够有效终止mRNA的转录。

4.能够利用URA3基因筛选带有ura3基因型的酵母宿主菌株转化子。

5.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。

6. 载体本身带有N端His和Xpress标签，同时载体C端也带有V5抗原表位和His纯化标签。

8. 在酵母中，利用载体上的2μ复制子维持质粒的高拷贝。

载体序列

LOCUS       pYES2/NT C	6037 bp 	DNA     circular	SYNDEFINITION  pYES2/NT CACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.COMMENT     This file is created by Vector NTI            http://www.biofeng.com/COMMENT     VNTAUTHORNAME|biofeng.com|FEATURES             Location/Qualifiers     source          1..6037                     /organism="pYES2/NT C"                     /mol_type="other DNA"     promoter        1..451                     /label="GAL1_promoter"     misc_feature    414..437                     /label="GAL1_primer"     promoter        475..493                     /label="T7_promoter"     misc_feature    542..560                     /label="Xpress_fwd_primer"     misc_feature    543..575                     /label="T7_leader"     misc_feature    579..602                     /label="Xpress_EK"     terminator      790..1035                     /label="CYC1_terminator"     misc_feature    complement(799..817)                     /label="CYC1_primer"     misc_feature    complement(799..817)                     /label="pYESTrp_rev_primer"     rep_origin      complement(1263..1882)                     /label="pBR322_origin"     CDS             complement(2037..2897)                     /label="ORF frame 3"     CDS             complement(2993..3796)                     /label="ORF frame 1"     gene            complement(2996..3796)                     /label="URA3"                     /gene="URA3"      CDS             3285..3761                     /label="ORF frame 3"     promoter        complement(3798..4023)                     /label="URA3_promoter"     rep_origin      4026..5497                     /label="2micron_origin"ORIGIN    1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT   61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA  121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC  181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA  241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT  301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC  361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC  421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC  481 GACTCACTAT AGGGAATATT AAGCTTACCA TGGGGGGTTC TCATCATCAT CATCATCATG  541 GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA  601 AGGTACCCGG ATCCAGTGTG GTGGAATTCT GCAGATATCC AGCACAGTGG CGGCCGCTCG  661 AGTCTAGAGG GCCCTTCGAA GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA  721 CGCGTACCGG TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCC TAGAGGGCCG  781 CATCATGTAA TTAGTTATGT CACGCTTACA TTCACGCCCT CCCCCCACAT CCGCTCTAAC  841 CGAAAAGGAA GGAGTTAGAC AACCTGAAGT CTAGGTCCCT ATTTATTTTT TTATAGTTAT  901 GTTAGTATTA AGAACGTTAT TTATATTTCA AATTTTTCTT TTTTTTCTGT ACAGACGCGT  961 GTACGCATGT AACATTATAC TGAAAACCTT GCTTGAGAAG GTTTTGGGAC GCTCGAAGGC 1021 TTTAATTTGC AAGCTGCGGC CCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT 1081 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 1141 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 1201 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGCCCAGGAA CCGTAAAAAG 1261 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 1321 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 1381 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 1441 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 1501 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 1561 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 1621 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 1681 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 1741 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 1801 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 1861 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 1921 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 1981 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 2041 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 2101 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGC GCTTACCATC TGGCCCCAGT 2161 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 2221 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 2281 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 2341 GTTGGCATTG CTACAGGCAT CGTGGTGTCA CTCTCGTCGT TTGGTATGGC TTCATTCAGC 2401 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 2461 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 2521 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 2581 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 2641 TGCCCGGCGT CAATACGGGA TAATAGTGTA TCACATAGCA GAACTTTAAA AGTGCTCATC 2701 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 2761 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 2821 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 2881 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATGGGTAA TAACTGATAT AATTAAATTG 2941 AAGCTCTAAT TTGTGAGTTT AGTATACATG CATTTACTTA TAATACAGTT TTTTAGTTTT 3001 GCTGGCCGCA TCTTCTCAAA TATGCTTCCC AGCCTGCTTT TCTGTAACGT TCACCCTCTA 3061 CCTTAGCATC CCTTCCCTTT GCAAATAGTC CTCTTCCAAC AATAATAATG TCAGATCCTG 3121 TAGAGACCAC ATCATCCACG GTTCTATACT GTTGACCCAA TGCGTCTCCC TTGTCATCTA 3181 AACCCACACC GGGTGTCATA ATCAACCAAT CGTAACCTTC ATCTCTTCCA CCCATGTCTC 3241 TTTGAGCAAT AAAGCCGATA ACAAAATCTT TGTCGCTCTT CGCAATGTCA ACAGTACCCT 3301 TAGTATATTC TCCAGTAGAT AGGGAGCCCT TGCATGACAA TTCTGCTAAC ATCAAAAGGC 3361 CTCTAGGTTC CTTTGTTACT TCTTCTGCCG CCTGCTTCAA ACCGCTAACA ATACCTGGGC 3421 CCACCACACC GTGTGCATTC GTAATGTCTG CCCATTCTGC TATTCTGTAT ACACCCGCAG 3481 AGTACTGCAA TTTGACTGTA TTACCAATGT CAGCAAATTT TCTGTCTTCG AAGAGTAAAA 3541 AATTGTACTT GGCGGATAAT GCCTTTAGCG GCTTAACTGT GCCCTCCATG GAAAAATCAG 3601 TCAAGATATC CACATGTGTT TTTAGTAAAC AAATTTTGGG ACCTAATGCT TCAACTAACT 3661 CCAGTAATTC CTTGGTGGTA CGAACATCCA ATGAAGCACA CAAGTTTGTT TGCTTTTCGT 3721 GCATGATATT AAATAGCTTG GCAGCAACAG GACTAGGATG AGTAGCAGCA CGTTCCTTAT 3781 ATGTAGCTTT CGACATGATT TATCTTCGTT TCCTGCAGGT TTTTGTTCTG TGCAGTTGGG 3841 TTAAGAATAC TGGGCAATTT CATGTTTCTT CAACACTACA TATGCGTATA TATACCAATC 3901 TAAGTCTGTG CTCCTTCCTT CGTTCTTCCT TCTGTTCGGA GATTACCGAA TCAAAAAAAT 3961 TTCAAAGAAA CCGAAATCAA AAAAAAGAAT AAAAAAAAAA TGATGAATTG AATTGAAAAG 4021 CTAGCTTATC GATGATAAGC TGTCAAAGAT GAGAATTAAT TCCACGGACT ATAGACTATA 4081 CTAGATACTC CGTCTACTGT ACGATACACT TCCGCTCAGG TCCTTGTCCT TTAACGAGGC 4141 CTTACCACTC TTTTGTTACT CTATTGATCC AGCTCAGCAA AGGCAGTGTG ATCTAAGATT 4201 CTATCTTCGC GATGTAGTAA AACTAGCTAG ACCGAGAAAG AGACTAGAAA TGCAAAAGGC 4261 ACTTCTACAA TGGCTGCCAT CATTATTATC CGATGTGACG CTGCAGCTTC TCAATGATAT 4321 TCGAATACGC TTTGAGGAGA TACAGCCTAA TATCCGACAA ACTGTTTTAC AGATTTACGA 4381 TCGTACTTGT TACCCATCAT TGAATTTTGA ACATCCGAAC CTGGGAGTTT TCCCTGAAAC 4441 AGATAGTATA TTTGAACCTG TATAATAATA TATAGTCTAG CGCTTTACGG AAGACAATGT 4501 ATGTATTTCG GTTCCTGGAG AAACTATTGC ATCTATTGCA TAGGTAATCT TGCACGTCGC 4561 ATCCCCGGTT CATTTTCTGC GTTTCCATCT TGCACTTCAA TAGCATATCT TTGTTAACGA 4621 AGCATCTGTG CTTCATTTTG TAGAACAAAA ATGCAACGCG AGAGCGCTAA TTTTTCAAAC 4681 AAAGAATCTG AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAAAGCGC TATTTTACCA 4741 ACGAAGAATC TGTGCTTCAT TTTTGTAAAA CAAAAATGCA ACGCGACGAG AGCGCTAATT 4801 TTTCAAACAA AGAATCTGAG CTGCATTTTT ACAGAACAGA AATGCAACGC GAGAGCGCTA 4861 TTTTACCAAC AAAGAATCTA TACTTCTTTT TTGTTCTACA AAAATGCATC CCGAGAGCGC 4921 TATTTTTCTA ACAAAGCATC TTAGATTACT TTTTTTCTCC TTTGTGCGCT CTATAATGCA 4981 GTCTCTTGAT AACTTTTTGC ACTGTAGGTC CGTTAAGGTT AGAAGAAGGC TACTTTGGTG 5041 TCTATTTTCT CTTCCATAAA AAAAGCCTGA CTCCACTTCC CGCGTTTACT GATTACTAGC 5101 GAAGCTGCGG GTGCATTTTT TCAAGATAAA GGCATCCCCG ATTATATTCT ATACCGATGT 5161 GGATTGCGCA TACTTTGTGA ACAGAAAGTG ATAGCGTTGA TGATTCTTCA TTGGTCAGAA 5221 AATTATGAAC GGTTTCTTCT ATTTTGTCTC TATATACTAC GTATAGGAAA TGTTTACATT 5281 TTCGTATTGT TTTCGATTCA CTCTATGAAT AGTTCTTACT ACAATTTTTT TGTCTAAAGA 5341 GTAATACTAG AGATAAACAT AAAAAATGTA GAGGTCGAGT TTAGATGCAA GTTCAAGGAG 5401 CGAAAGGTGG ATGGGTAGGT TATATAGGGA TATAGCACAG AGATATATAG CAAAGAGATA 5461 CTTTTGAGCA ATGTTTGTGG AAGCGGTATT CGCAATGGGA AGCTCCACCC CGGTTGATAA 5521 TCAGAAAAGC CCCAAAAACA GGAAGATTGT ATAAGCAAAT ATTTAAATTG TAAACGTTAA 5581 TATTTTGTTA AAATTCGCGT TAAATTTTTG TTAAATCAGC TCATTTTTTA ACGAATAGCC 5641 CGAAATCGGC AAAATCCCTT ATAAATCAAA AGAATAGACC GAGATAGGGT TGAGTGTTGT 5701 TCCAGTTTCC AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA 5761 AAGGGTCTAT CAGGGCGATG GCCCACTACG TGAACCATCA CCCTAATCAA GTTTTTTGGG 5821 GTCGAGGTGC CGTAAAGCAG TAAATCGGAA GGGTAAACGG ATGCCCCCAT TTAGAGCTTG 5881 ACGGGGAAAG CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGGGC 5941 TAGGGCGGTG GGAAGTGTAG GGGTCACGCT GGGCGTAACC ACCACACCCG CCGCGCTTAA 6001 TGGGGCGCTA CAGGGCGCGT GGGGATGATC CACTAGT