pUB6/V5 His B

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pUB6/V5 His B

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pUB6/V5 His B

pUB6/V5HisB载体基本信息

出品公司:	Ybscience
载体名称:	pUB6/V5 His B
质粒类型:	哺乳细胞表达系统载体
高拷贝/低拷贝:	高拷贝
启动子:	UbC
克隆方法:	多克隆位点，限制性内切酶
载体大小:	5467bp
5' 测序引物及序列:	T7 Fwd：5'd[TAATACGACTCACTATAGGG]3'
3' 测序引物及序列:	--
载体标签:	6X His, V5
载体抗性:	Ampicillin
筛选标记:	Blasticidin
备注:	可以严格控制表达水平
稳定性:	瞬表达
组成型:	组成型
病毒/非病毒:	非病毒

 

pUB6/V5HisB载体质粒图谱和多克隆位点信息

 

pUB6/V5HisB载体简介

 

pUB6/V5HisB载体序列

LOCUS       pUB6/V5/His B	5467 bp 	DNA	SYNDEFINITION  pUB6/V5/His BACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..5467                     /organism="pUB6/V5/His B"                     /mol_type="other DNA"     promoter        13..1227                     /label="hUbC_promoter"     misc_feature    complement(1422..1439)                     /label="BGH_rev_primer"     terminator      1425..1652                     /label="bGH_PA_terminator"     rep_origin      1715..2021                     /label="f1_origin"     misc_feature    complement(2135..2155)                     /label="pBABE_3_primer"     misc_feature    complement(2141..2357)                     /label="SV40_enhancer"     promoter        2153..2422                     /label="SV40_promoter"     rep_origin      2321..2398                     /label="SV40_origin"     misc_feature    2383..2402                     /label="SV40pro_F_primer"     promoter        2516..2583                     /label="EM7_promoter"     gene            2584..2982                     /label="blast"                     /gene="blast"     terminator      3143..3262                     /label="SV40_PA_terminator"     misc_feature    3231..3250                     /label="EBV_rev_primer"     promoter        complement(3306..3324)                     /label="M13_reverse_primer"     misc_feature    complement(3323..3345)                     /label="M13_pUC_rev_primer"     promoter        complement(3359..3388)                     /label="lac_promoter"     rep_origin      complement(3697..4316)                     /label="pBR322_origin"     gene            complement(4471..5331)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(4471..5331)                     /label="ORF frame 2"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        complement(5373..5401)                     /label="AmpR_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 GACGGATCGG GAGATCTGGC CTCCGCGCCG GGTTTTGGCG CCTCCCGCGG GCGCCCCCCT   61 CCTCACGGCG AGCGCTGCCA CGTCAGACGA AGGGCGCAGG AGCGTCCTGA TCCTTCCGCC  121 CGGACGCTCA GGACAGCGGC CCGCTGCTCA TAAGACTCGG CCTTAGAACC CCAGTATCAG  181 CAGAAGGACA TTTTAGGACG GGACTTGGGT GACTCTAGGG CACTGGTTTT CTTTCCAGAG  241 AGCGGAACAG GCGAGGAAAA GTAGTCCCTT CTCGGCGATT CTGCGGAGGG ATCTCCGTGG  301 GGCGGTGAAC GCCGATGATT ATATAAGGAC GCGCCGGGTG TGGCACAGCT AGTTCCGTCG  361 CAGCCGGGAT TTGGGTCGCG GTTCTTGTTT GTGGATCGCT GTGATCGTCA CTTGGTGAGT  421 AGCGGGCTGC TGGGCTGGCC GGGGCTTTCG TGGCCGCCGG GCCGCTCGGT GGGACGGAAG  481 CGTGTGGAGA GACCGCCAAG GGCTGTAGTC TGGGTCCGCG AGCAAGGTTG CCCTGAACTG  541 GGGGTTGGGG GGAGCGCAGC AAAATGGCGG CTGTTCCCGA GTCTTGAATG GAAGACGCTT  601 GTGAGGCGGG CTGTGAGGTC GTTGAAACAA GGTGGGGGGC ATGGTGGGCG GCAAGAACCC  661 AAGGTCTTGA GGCCTTCGCT AATGCGGGAA AGCTCTTATT CGGGTGAGAT GGGCTGGGGC  721 ACCATCTGGG GACCCTGACG TGAAGTTTGT CACTGACTGG AGAACTCGGT TTGTCGTCTG  781 TTGCGGGGGC GGCAGTTATG CGGTGCCGTT GGGCAGTGCA CCCGTACCTT TGGGAGCGCG  841 CGCCCTCGTC GTGTCGTGAC GTCACCCGTT CTGTTGGCTT ATAATGCAGG GTGGGGCCAC  901 CTGCCGGTAG GTGTGCGGTA GGCTTTTCTC CGTCGCAGGA CGCAGGGTTC GGGCCTAGGG  961 TAGGCTCTCC TGAATCGACA GGCGCCGGAC CTCTGGTGAG GGGAGGGATA AGTGAGGCGT 1021 CAGTTTCTTT GGTCGGTTTT ATGTACCTAT CTTCTTAAGT AGCTGAAGCT CCGGTTTTGA 1081 ACTATGCGCT CGGGGTTGGC GAGTGTGTTT TGTGAAGTTT TTTAGGCACC TTTTGAAATG 1141 TAATCATTTG GGTCAATATG TAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC 1201 TGGCCGTTTT TGGCTTTTTT GTTAGACGAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC 1261 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC 1321 GCGGTTCGAA GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGCGTACCGG 1381 TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCA GCCTCGACTG TGCCTTCTAG 1441 TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC TTGACCCTGG AAGGTGCCAC 1501 TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG CATTGTCTGA GTAGGTGTCA 1561 TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG AAGACAATAG 1621 CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA CCAGCTGGGG 1681 CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT 1741 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT 1801 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC 1861 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA 1921 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC 1981 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT 2041 CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT 2101 GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 2161 AAGTCCCCAG GCTCCCCAGG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 2221 AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT 2281 CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC 2341 CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA 2401 GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 2461 CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AGCACGTGTT 2521 GACAATTAAT CATCGGCATA GTATATCGGC ATAGTATAAT ACGACAAGGT GAGGAACTAA 2581 ACCATGGCCA AGCCTTTGTC TCAAGAAGAA TCCACCCTCA TTGAAAGAGC AACGGCTACA 2641 ATCAACAGCA TCCCCATCTC TGAAGACTAC AGCGTCGCCA GCGCAGCTCT CTCTAGCGAC 2701 GGCCGCATCT TCACTGGTGT CAATGTATAT CATTTTACTG GGGGACCTTG TGCAGAACTC 2761 GTGGTGCTGG GCACTGCTGC TGCTGCGGCA GCTGGCAACC TGACTTGTAT CGTCGCGATC 2821 GGAAATGAGA ACAGGGGCAT CTTGAGCCCC TGCGGACGGT GCCGACAGGT GCTTCTCGAT 2881 CTGCATCCTG GGATCAAAGC CATAGTGAAG GACAGTGATG GACAGCCGAC GGCAGTTGGG 2941 ATTCGTGAAT TGCTGCCCTC TGGTTATGTG TGGGAGGGCT AAGCACTTCG TGGCCGAGGA 3001 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 3061 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 3121 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 3181 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 3241 CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC 3301 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 3361 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 3421 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 3481 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 3541 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 3601 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 3661 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 3721 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 3781 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 3841 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 3901 TTCTCAATGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 3961 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 4021 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 4081 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 4141 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 4201 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 4261 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 4321 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 4381 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 4441 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 4501 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 4561 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 4621 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 4681 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 4741 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 4801 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 4861 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 4921 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 4981 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 5041 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 5101 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 5161 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 5221 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 5281 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 5341 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 5401 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 5461 TGACGTC