pSV-Beta-Galactosidase

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pSV-Beta-Galactosidase

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pSV-Beta-Galactosidase

pSV-β-Gal载体基本信息

出品公司:	Ybscience
载体名称:	pSV-Beta-Gal
质粒类型:	哺乳细胞表达；Beta-Gal报告载体
高拷贝/低拷贝:	高拷贝
启动子:	SV40
克隆方法:	多克隆位点，限制性内切酶
载体大小:	6820 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	lacZ
载体抗性:	氨苄青霉素
筛选标记:	--
备注:	表达水平高
稳定性:	瞬表达
组成型:	组成型
病毒/非病毒:	非病毒

 

pSV-β-Gal载体质粒图谱和多克隆位点信息

 

pSV-β-Gal载体简介

 

pSV-β-Gal载体序列

LOCUS       pSV-beta-Galactosidase	6820 bp 	DNA  circular	SYNDEFINITION  pSV-beta-GalactosidaseACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..6820                     /organism="pSV-beta-Galactosidase"                     /mol_type="other DNA"     misc_feature    complement(79..99)                     /label="pBABE_3_primer"     misc_feature    complement(85..541)                     /label="SV40_enhancer"     promoter        97..365                     /label="SV40_promoter"     rep_origin      264..341                     /label="SV40_origin"     misc_feature    326..345                     /label="SV40pro_F_primer"     CDS             500..3757                     /label="ORF frame 2"     gene            710..3754                     /label="lacZ"                     /gene="lacZ"     misc_feature    complement(712..734)                     /label="M13_pUC_fwd_primer"     misc_feature    3809..4011                     /label="lacY"     CDS             3809..4438                     /label="ORF frame 2"     terminator      4024..4155                     /label="SV40_PA_terminator"     misc_feature    4112..4131                     /label="EBV_rev_primer"     promoter        complement(4189..4205)                     /label="M13_forward20_primer"     misc_feature    complement(4198..4220)                     /label="M13_pUC_fwd_primer"     misc_feature    4198..4341                     /label="lacZ_a"     misc_feature    4533..4555                     /label="pGEX_3_primer"     promoter        4714..4742                     /label="AmpR_promoter"     gene            4784..5644                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             4784..5644                     /label="ORF frame 2"     rep_origin      5799..6418                     /label="pBR322_origin"     promoter        6727..6756                     /label="lac_promoter"     misc_feature    6770..6792                     /label="M13_pUC_rev_primer"ORIGIN    1 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT ACCTTCTGAG   61 GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC  121 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT  181 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA  241 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC  301 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG  361 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTG  421 GGATCTCTAT AATCTCGCGC AACCTATTTT CCCCTCGAAC ACTTTTTAAG CCGTAGATAA  481 ACAGGCTGGG ACACTTCACA TGAGCGAAAA ATACATCGTC ACCTGGGACA TGTTGCAGAT  541 CCATGCACGT AAACTCGCAA GCCGACTGAT GCCTTCTGAA CAATGGAAAG GCATTATTGC  601 CGTAAGCCGT GGCGGTCTGG TACCGGTGGG TGAAGACCAG AAACAGCACC TCGAACTGAG  661 CCGCGATATT GCCCAGCGTT TCAACGCGCT GTATGGCGAG ATCGATCCCG TCGTTTTACA  721 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC  781 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG  841 CAGCCTGAAT GGCGAATGGC GCTTTGCCTG GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG  901 CTGGCTGGAG TGCGATCTTC CTGAGGCCGA TACTGTCGTC GTCCCCTCAA ACTGGCAGAT  961 GCACGGTTAC GATGCGCCCA TCTACACCAA CGTGACCTAT CCCATTACGG TCAATCCGCC 1021 GTTTGTTCCC ACGGAGAATC CGACGGGTTG TTACTCGCTC ACATTTAATG TTGATGAAAG 1081 CTGGCTACAG GAAGGCCAGA CGCGAATTAT TTTTGATGGC GTTAACTCGG CGTTTCATCT 1141 GTGGTGCAAC GGGCGCTGGG TCGGTTACGG CCAGGACAGT CGTTTGCCGT CTGAATTTGA 1201 CCTGAGCGCA TTTTTACGCG CCGGAGAAAA CCGCCTCGCG GTGATGGTGC TGCGCTGGAG 1261 TGACGGCAGT TATCTGGAAG ATCAGGATAT GTGGCGGATG AGCGGCATTT TCCGTGACGT 1321 CTCGTTGCTG CATAAACCGA CTACACAAAT CAGCGATTTC CATGTTGCCA CTCGCTTTAA 1381 TGATGATTTC AGCCGCGCTG TACTGGAGGC TGAAGTTCAG ATGTGCGGCG AGTTGCGTGA 1441 CTACCTACGG GTAACAGTTT CTTTATGGCA GGGTGAAACG CAGGTCGCCA GCGGCACCGC 1501 GCCTTTCGGC GGTGAAATTA TCGATGAGCG TGGTGGTTAT GCCGATCGCG TCACACTACG 1561 TCTGAACGTC GAAAACCCGA AACTGTGGAG CGCCGAAATC CCGAATCTCT ATCGTGCGGT 1621 GGTTGAACTG CACACCGCCG ACGGCACGCT GATTGAAGCA GAAGCCTGCG ATGTCGGTTT 1681 CCGCGAGGTG CGGATTGAAA ATGGTCTGCT GCTGCTGAAC GGCAAGCCGT TGCTGATTCG 1741 AGGCGTTAAC CGTCACGAGC ATCATCCTCT GCATGGTCAG GTCATGGATG AGCAGACGAT 1801 GGTGCAGGAT ATCCTGCTGA TGAAGCAGAA CAACTTTAAC GCCGTGCGCT GTTCGCATTA 1861 TCCGAACCAT CCGCTGTGGT ACACGCTGTG CGACCGCTAC GGCCTGTATG TGGTGGATGA 1921 AGCCAATATT GAAACCCACG GCATGGTGCC AATGAATCGT CTGACCGATG ATCCGCGCTG 1981 GCTACCGGCG ATGAGCGAAC GCGTAACGCG AATGGTGCAG CGCGATCGTA ATCACCCGAG 2041 TGTGATCATC TGGTCGCTGG GGAATGAATC AGGCCACGGC GCTAATCACG ACGCGCTGTA 2101 TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG TATGAAGGCG GCGGAGCCGA 2161 CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC GTGGATGAAG ACCAGCCCTT 2221 CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG CTACCTGGAG AGACGCGCCC 2281 GCTGATCCTT TGCGAATACG CCCACGCGAT GGGTAACAGT CTTGGCGGTT TCGCTAAATA 2341 CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC TTCGTCTGGG ACTGGGTGGA 2401 TCAGTCGCTG ATTAAATATG ATGAAAACGG CAACCCGTGG TCGGCTTACG GCGGTGATTT 2461 TGGCGATACG CCGAACGATC GCCAGTTCTG TATGAACGGT CTGGTCTTTG CCGACCGCAC 2521 GCCGCATCCA GCGCTGACGG AAGCAAAACA CCAGCAGCAG TTTTTCCAGT TCCGTTTATC 2581 CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT CATAGCGATA ACGAGCTCCT 2641 GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC GGTGAAGTGC CTCTGGATGT 2701 CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA CCGCAGCCGG AGAGCGCCGG 2761 GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG ACCGCATGGT CAGAAGCCGG 2821 GCACATCAGC GCCTGGCAGC AGTGGCGTCT GGCGGAAAAC CTCAGTGTGA CGCTCCCCGC 2881 CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG GATTTTTGCA TCGAGCTGGG 2941 TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT TCACAGATGT GGATTGGCGA 3001 TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC CGTGCACCGC TGGATAACGA 3061 CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC TGGGTCGAAC GCTGGAAGGC 3121 GGCGGGCCAT TACCAGGCCG AAGCAGCGTT GTTGCAGTGC ACGGCAGATA CACTTGCTGA 3181 TGCGGTGCTG ATTACGACCG CTCACGCGTG GCAGCATCAG GGGAAAACCT TATTTATCAG 3241 CCGGAAAACC TACCGGATTG ATGGTAGTGG TCAAATGGCG ATTACCGTTG ATGTTGAAGT 3301 GGCGAGCGAT ACACCGCATC CGGCGCGGAT TGGCCTGAAC TGCCAGCTGG CGCAGGTAGC 3361 AGAGCGGGTA AACTGGCTCG GATTAGGGCC GCAAGAAAAC TATCCCGACC GCCTTACTGC 3421 CGCCTGTTTT GACCGCTGGG ATCTGCCATT GTCAGACATG TATACCCCGT ACGTCTTCCC 3481 GAGCGAAAAC GGTCTGCGCT GCGGGACGCG CGAATTGAAT TATGGCCCAC ACCAGTGGCG 3541 CGGCGACTTC CAGTTCAACA TCAGCCGCTA CAGTCAACAG CAACTGATGG AAACCAGCCA 3601 TCGCCATCTG CTGCACGCGG AAGAAGGCAC ATGGCTGAAT ATCGACGGTT TCCATATGGG 3661 GATTGGTGGC GACGACTCCT GGAGCCCGTC AGTATCGGCG GAATTCCAGC TGAGCGCCGG 3721 TCGCTACCAT TACCAGTTGG TCTGGTGTCA AAAATAATAA TAACCGGGCA GGCCATGTCT 3781 GCCCGTATTT CGCGTAAGGA AATCCATTAT GTACTATTTA AAAAACACAA ACTTTTGGAT 3841 GTTCGGTTTA TTCTTTTTCT TTTACTTTTT TATCATGGGA GCCTACTTCC CGTTTTTCCC 3901 GATTTGGCTA CATGACATCA ACCATATCAG CAAAAGTGAT ACGGGTATTA TTTTTGCCGC 3961 TATTTCTCTG TTCTCGCTAT TATTCCAACC GCTGTTTGGT CTGCTTTCTG ACAAACTCGG 4021 AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA 4081 AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT 4141 TATCATGTCT GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTGGCAC TGGCCGTCGT 4201 TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA 4261 TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA 4321 GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG 4381 CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT 4441 AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC 4501 GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC 4561 ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT 4621 TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG 4681 CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA 4741 ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT 4801 CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA 4861 AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA 4921 ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT 4981 GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA 5041 AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT 5101 CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC 5161 CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT 5221 AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA 5281 GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC 5341 AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT 5401 AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG 5461 CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC 5521 ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC 5581 AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG 5641 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA 5701 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG 5761 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA 5821 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT 5881 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG 5941 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA 6001 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG 6061 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA 6121 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC 6181 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA 6241 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC 6301 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG 6361 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC 6421 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC 6481 CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG 6541 CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA 6601 ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA GGTTTCCCGA 6661 CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTGAGT TAGCTCACTC ATTAGGCACC 6721 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA GCGGATAACA 6781 ATTTCACACA GGAAACAGCT ATGACATGAT TACGAATTCG