pCMV-LacZ

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCMV-LacZ

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCMV-LacZ

体基本信息

出品公司:	Ybscience
载体名称:	pCMV-LacZ
质粒类型:	哺乳动物表达载体；β-gal报告载体
高拷贝/低拷贝:	高拷贝
克隆方法:	--
启动子:	CMV
载体大小:	7.2 kb
5' 测序引物及序列:	CMV Forward CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	无
克隆菌株:	DH5a 或 HB101
宿主细胞（系）:	--
备注:	pCMV-LacZ载体是β半乳糖甘酶表达载体，在哺乳动物细胞中高水平表达β半乳糖甘酶； 可用于建立标准浓度或者标准菌株，定量分析其它报告型载体的表达情况。
稳定性:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

pCMV-LacZ is a mammalian reporter vector designed to expression β-galactosidase in mammalian cells from the human cytomegalovirus immediate early gene promoter (1). pCMV-LacZ contains an intron (splice donor/splice acceptor; 2) and polyadenylation signal from SV40, and the full-length E. coli β-galactosidase gene with eukaryotic translation initiation signals (3). pCMV-LacZ expresses high levels of β-galactosidase and can be used as a reference (control) plasmid when transfecting other reporter gene constructs and can be used to optimize transfection protocols by employing standard assays or stains to assay β-galactosidase activity. Alternatively, the β-galactosidase gene can be excised using the Not I sites at each end to allow other genes to be inserted into the pCMV-LacZ vector backbone for expression in mammalian cells or to insert the β-galactosidase fragment into another expression vector.