pCMV-MEKK1

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCMV-MEKK1

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCMV-MEKK1

载体基本信息

出品公司:	Ybscience
载体名称:	pCMV-MEKK1
质粒类型:	哺乳动物表达载体；基因表达载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	5 kb
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	无
载体抗性:	卡那霉素
筛选标记:	Neomycin
克隆菌株:	TOP10F′, JM109 and INVaF′
宿主细胞（系）:	常规细胞系，如293、Hela等
备注:	pCMV-MEKK1载体是蛋白激酶MEKK1的表达载体； CMV启动子驱动MEKK1基因的过表达； MEKK1蛋白激酶是JNK/SAPK信号通路的应答激酶。
稳定性:	瞬表达 或 稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

pCMV-MEKK1载体使用说明:pCMV-MEKK1 is designed for the constitutive expression of MEK Kinase 1 (MEKK1) in mammalian cells. It can also be used to study the effects of a given stimulus on the JNK/SAPK pathway. In conjunction with Clontech’s In Vivo Kinase Assay Kits, pCMV-MEKK1 is an ideal positive control for studying a gene or molecule and its affect on kinase activation in the JNK/SAPK pathway.MEKK1 mediates cellular responses to diverse stimuli, such as cytokines, growth factors, and other stressors via activation of JNK/SAPK signaling pathway. It has also been reported that MEKK1 is a possible regulator in signal transduction pathways involving apoptosis (1). To a lesser extent, MEKK1 can also activate the ERK pathway via phosphorylation of MEK (2, 3); however, it is more efficient in activating the JNK/SAPK than ERK (4, 5).pCMV-MEKK1 contains a gene encoding MEKK1 (mouse) that is driven by the human cytomegalovirus(CMV) promoter. SV40 polyadenylation signals downstream of the MEKK1 gene direct proper processing of the 3’ end of the mRNA. The vector backbone also contains an SV40 origin for replication in mammalian cells expressing the SV40 T-antigen. A neomycin resistance cassette (Neor)—consisting of the SV40 early promoter, the neomycin/kanamycin resistance gene of Tn5, and polyadenylation signals from the Herpes simplex virus thymidine kinase (HSV TK) gene—allows stably transfected eukaryotic cells to be selected using G418(6). A bacterial promoter upstream of this cassette expresses kanamycin resistance in E. coli. The pCMV-MEKK1 backbone also provides a pUC origin of replication for propagation in E.coli and an f1 origin for single-stranded DNA production. The recombinant vector can be transfected into mammalian cells using any standard method.Propagation in E. coli: Suitable host strains: DH5α, HB101, and other general purpose strains. Single-stranded DNA production requiresa host containing an F plasmid such as JM109 or XL1-Blue. Selectable marker: plasmid confers resistance to kanamycin (50 μg/ml) to E. coli hosts. E. coli replication origin: pUC Copy number: ≈500 Plasmid incompatibility group: pMB1/ColE1