pCMV-RFP-C-Myc

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCMV-RFP-C-Myc

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCMV-RFP-C-Myc

载体基本信息

出品公司:	Ybscience
载体名称:	pCMV-RFP-C-Myc
质粒类型:	哺乳动物表达载体；荧光报告载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	4726 bp
5' 测序引物及序列:	CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列:	BGH rev 5''TAGAAGGCACAGTCGAGG3''
载体标签:	RFP、Myc (C ter）
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	DH5α 等
宿主细胞（系）:	哺乳动物细胞
备注:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       Exported                4726 bp ds-DNA     circular SYN 12-AUG-2016KEYWORDS    pCMV-RFP-C-MycSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 4726)  AUTHORS   Biofeng Lab.FEATURES             Location/Qualifiers     source          1..4726                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     enhancer        67..370                     /note="CMV enhancer"                     /note="human cytomegalovirus immediate early enhancer"     promoter        371..618                     /note="CMV promoter"                     /note="human cytomegalovirus (CMV) immediate early                      promoter"     promoter        625..643                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"     misc_feature    650..652                     /note="ATG"     misc_feature    659..696                     /note="MCS"     gene            707..1399                     /note="RFP"     CDS             1400..1429                     /codon_start=1                     /product="Myc (human c-Myc oncogene) epitope tag"                     /note="Myc"                     /translation="EQKLISEEDL"     polyA_signal    1459..1584                     /note="bGH poly(A)"                     /note="bovine growth hormone polyadenylation signal"     rep_origin      complement(1642..2097)                     /direction=LEFT                     /note="f1 ori"                     /note="f1 bacteriophage origin of replication; arrow                      indicates direction of (+) strand synthesis"     promoter        2124..2228                     /gene="bla"                     /note="AmpR promoter"     promoter        2328..2587                     /note="SV40 promoter"                     /note="SV40 enhancer and early promoter"     rep_origin      2438..2573                     /note="SV40 ori"                     /note="SV40 origin of replication"     CDS             2622..3416                     /codon_start=1                     /gene="aph(3')-II (or nptII)"                     /product="aminoglycoside phosphotransferase from Tn5"                     /note="NeoR/KanR"                     /note="confers resistance to neomycin, kanamycin, and G418                      (Geneticin(R))"                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"     polyA_signal    3648..3695                     /note="HSV TK poly(A)"                     /note="herpes simplex virus thymidine kinase                      polyadenylation signal (Cole and Stacy, 1985)"     rep_origin      4001..4644                     /direction=RIGHT                     /note="pUC ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"ORIGIN        1 tagttattaa tcccgggagt aatcaattac ggggtcatta gttcatagcc catatatgga       61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag      541 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc      601 ttactggctt atcgaaattc tagataatac gactcactat agggcgaata tggccacggg      661 cgcgccctgc aggctagcct cgagggatcc gatatcgcgg ccgcttcttt ccaaacaagt      721 tttgcctcat gatgtgaaaa tgagatacca tatggatgga tgtgttaacg gtcacagttt      781 cactattgag ggagaaggtg ctggtaaacc ttatgaaggc aaaaagatct tggaactgcg      841 tgtaacaaag ggaggaccac ttccattcgc ctttgacatt ttgtccagcg ttttcactta      901 tggtaacagg tgtttctgtg agtatccaga ggatatgccc gactacttta agcaatcatt      961 gcccgagggt cactcttggg agagaacttt aatgtttgag gatggaggat gcggtactgc     1021 ttcagcccat atatcgttgg acaaaaactg tttcgtgcat aagtctacgt ttcatggtgt     1081 gaattttcct gctaatggtc cggtcatgca gaaaaaggca atgaattggg agcctagctc     1141 tgaactgatc accgcatgtg atggtattct caaaggcgac gttaccatgt ttttgttgct     1201 ggaaggtggt cacagattaa agtgccaatt cacaacatcc tacaaggctc ataaagcagt     1261 gaagatgcca ccaaatcaca ttatcgagca tgtcctagtc aaaaaggaag ttgctgacgg     1321 ttttcaaatc caggaacacg ctgttgcaaa acacttcaca gtagatgtta aggaaactcg     1381 cggccgcgaa ttcagatctg agcagaagct gatctccgag gaggacctgg ttaacttatg     1441 ataaggccgg cctgatcaaa aatcagcctc gactgtgcct tctagttgcc agccatctgt     1501 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc     1561 ctaataaaat gaggaaattg catctgtaca tctagcattc agttgtggtt tgtccaaact     1621 catcaattta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa     1681 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata     1741 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac     1801 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc     1861 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa     1921 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg     1981 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg     2041 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag     2101 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt     2161 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa     2221 ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa     2281 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac     2341 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa     2401 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag     2461 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc     2521 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt     2581 ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat     2641 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac     2701 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc     2761 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc     2821 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag     2881 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc     2941 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg     3001 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc     3061 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc     3121 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga     3181 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca     3241 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg     3301 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg     3361 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg     3421 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga     3481 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg     3541 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct     3601 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag     3661 acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg     3721 ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc     3781 cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg     3841 ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact     3901 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat     3961 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc     4021 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct     4081 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg     4141 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca     4201 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc     4261 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga     4321 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac     4381 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga     4441 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag     4501 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg     4561 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag     4621 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc     4681 tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat