pCMV-SPORT2

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCMV-SPORT2

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCMV-SPORT2

载体基本信息

出品公司:	Ybscience
载体名称:	pCMV-SPORT2
质粒类型:	哺乳动物表达载体；cDNA克隆载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	4774 bp
5' 测序引物及序列:	CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列:	SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	DH5α 等
宿主细胞（系）:	哺乳动物细胞
稳定性:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pCMV-Sport2	4474 bp 	DNA	SYNDEFINITION  pCMV-Sport2ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..4474                     /organism="pCMV-Sport2"                     /mol_type="other DNA"     terminator      complement(135..254)                     /label="SV40_PA_terminator"     misc_feature    complement(149..168)                     /label="EBV_rev_primer"     misc_feature    complement(542..557)                     /label="SV40_int"     misc_feature    649..671                     /label="M13_pUC_fwd_primer"     promoter        664..680                     /label="M13_forward20_primer"     promoter        691..709                     /label="T7_promoter"     misc_feature    complement(833..850)                     /label="Sp6_primer"     misc_feature    complement(886..908)                     /label="M13_pUC_rev_primer"     promoter        complement(932..1051)                     /label="CMV2_promoter"     misc_feature    complement(994..1018)                     /label="LNCX_primer"     promoter        complement(994..1065)                     /label="CMV_promoter"     misc_feature    complement(1001..1020)                     /label="pCEP_fwd_primer"     promoter        complement(1017..1521)                     /label="CMV_immearly_promoter"     misc_feature    complement(1044..1064)                     /label="CMV_fwd_primer"     misc_feature    complement(1230..1517)                     /label="CAG_enhancer"     misc_feature    complement(1541..1563)                     /label="M13_pUC_rev_primer"     promoter        1754..1782                     /label="AmpR_promoter"     rep_origin      complement(2054..2673)                     /label="pBR322_origin"     misc_feature    2735..2768                     /label="loxP"     gene            complement(2870..3730)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(2870..3730)                     /label="ORF frame 1"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     rep_origin      complement(4150..4456)                     /label="f1_origin"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct   61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag  121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca  181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat  241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg  301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta  361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct  421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact  481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta  541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca  601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagatctcc cagtcacgac  661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg agagagctat  721 gacgtcgcat gcacgcgtaa gcttgggccc ctcgagggat cctctagagc ggccgccgac  781 tagtgagctc gtcgacccgg gaattccgga ccggtacctg caggcgtacc ttctatagtg  841 tcacctaaat agctttttgc aaaagcctag tggtcatagc tgtttcctgt gtgaaattgt  901 tatccgctcc gcggcctagg ctagagtccg gaggctggat cggtcccggt gtcttctatg  961 gaggtcaaaa cagcgtggat ggcgtctcca ggcgatctga cggttcacta aacgagctct 1021 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt 1081 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc 1141 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta 1201 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt 1261 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt 1321 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt 1381 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg 1441 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc 1501 atttaccgta agttatgtaa cgacctgcac gatgctgttt cctgtgtgaa attgttatcc 1561 gctcacaatt ccacacatta tacgagccgg aagctataaa gtgtaaagcc tggggtgcct 1621 aatgagtgaa agggcctcgt atacgcctat ttttataggt taatgtcatg ataataatgg 1681 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 1741 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 1801 aataatattg aaaaacgcgc gaattgcaag ctctgcatta atgaatcggc caacgcgcgg 1861 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 1921 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 1981 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 2041 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 2101 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 2161 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 2221 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 2281 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 2341 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 2401 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 2461 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 2521 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 2581 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 2641 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 2701 acgaaaactc acgttaaggg attttggtca tgccataact tcgtatagca tacattatac 2761 gaagttatgg catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 2821 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 2881 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 2941 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 3001 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 3061 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 3121 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 3181 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 3241 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 3301 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 3361 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 3421 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 3481 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 3541 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 3601 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 3661 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 3721 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 3781 tgccaggggt gggcacacat atttgatacc agcgatccct acacagcaca taattcaatg 3841 cgacttccct ctatcgcaca tcttagacct ttattctccc tccagcacac atcgaagctg 3901 ccgagcaagc cgttctcacc agtccaagac ctggcatgag cggatacata tttgaatgta 3961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgaaa 4021 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt 4081 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag 4141 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg 4201 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat 4261 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc 4321 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga 4381 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac 4441 ccgccgcgct taatgcgccg ctacagggcg cgtc