pCMV-SPORT6.1

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCMV-SPORT6.1

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCMV-SPORT6.1

载体基本信息

出品公司:	Ybscience
载体名称:	pCMV-SPORT6.1
质粒类型:	哺乳动物表达载体；cDNA克隆载体；I.M.A.G.E.载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点；Gateway载体
启动子:	CMV
载体大小:	4177 bp
5' 测序引物及序列:	CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列:	SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	DH5α 等
宿主细胞（系）:	哺乳动物细胞
备注:	pCMV-SPORT6.1有多个限制性酶切位点，又可用于Gateway克隆，用于cDNA文库的构建和表达。
稳定性:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       Exported                4177 bp ds-DNA     circular SYN 12-AUG-2016DEFINITION  Gateway(R) library vector for cloning and transient mammalian cell             expression of cDNAs. See pCMV SPORT6 for an older version of this             vector.KEYWORDS    pCMV SPORT6.1SOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 4177)  AUTHORS   Invitrogen (Life Technologies)COMMENT     The cDNA is inserted between bases 781-782, destroying an EcoRV site            present in the original vector.FEATURES             Location/Qualifiers     source          1..4177                     /organism="synthetic DNA construct"                     /lab_host="Mammalian Cells"                     /mol_type="other DNA"     polyA_signal    125..259                     /note="SV40 poly(A) signal"                     /note="SV40 polyadenylation signal"     primer_bind     664..680                     /note="M13 fwd"                     /note="common sequencing primer, one of multiple similar                      variants"     promoter        691..709                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"     protein_bind    711..735                     /gene="mutant version of attB"                     /bound_moiety="BP Clonase(TM)"                     /note="attB2"                     /note="recombination site for the Gateway(R) BP reaction"     misc_feature    736..806                     /note="MCS"     protein_bind    complement(807..831)                     /gene="mutant version of attB"                     /bound_moiety="BP Clonase(TM)"                     /note="attB1"                     /note="recombination site for the Gateway(R) BP reaction"     promoter        complement(832..850)                     /note="SP6 promoter"                     /note="promoter for bacteriophage SP6 RNA polymerase"     primer_bind     complement(861..877)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"     promoter        complement(1003..1206)                     /note="CMV promoter"                     /note="human cytomegalovirus (CMV) immediate early                      promoter"     enhancer        1207..1510                     /note="CMV enhancer"                     /note="human cytomegalovirus immediate early enhancer"     rep_origin      complement(1970..2558)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     CDS             complement(2729..3589)                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /note="AmpR"                     /note="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     promoter        complement(3590..3694)                     /gene="bla"                     /note="AmpR promoter"     rep_origin      complement(3721..4176)                     /direction=LEFT                     /note="f1 ori"                     /note="f1 bacteriophage origin of replication; arrow                      indicates direction of (+) strand synthesis"ORIGIN        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac      661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactttgt      721 acaagaaagc tgggtacgcg taagcttggg cccctcgagg gatactctag agcggccgcc      781 catcccggga attccggacc ggtaccagcc tgcttttttg tacaaacttg ttctatagtg      841 tcacctaaat aggcctaatg gtcatagctg tttcctgtgt gaaattgtta tccgctccgc      901 ggcctaggct agagtccgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca      961 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac     1021 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt     1081 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag     1141 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg     1201 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca     1261 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg     1321 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata     1381 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat     1441 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag     1501 ttatgtaacg acatgcatct aatgagtgaa agggcctcgt actacgccta tttttatagg     1561 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc     1621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac     1681 aataaccctg ataaatgctt caataatatt gaaaaacgcg cgaattgcaa gctctgcatt     1741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct     1801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa     1861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa     1921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc     1981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga     2041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     2101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     2161 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     2221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     2281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     2341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     2401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa     2461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     2521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta     2581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat     2641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa     2701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct     2761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta     2821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct     2881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg     2941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa     3001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt     3061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta     3121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca     3181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta     3241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct     3301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg     3361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac     3421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact     3481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa     3541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt     3601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat     3661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg     3721 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat     3781 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga     3841 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca     3901 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct     3961 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc     4021 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag     4081 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca     4141 cacccgccgc gcttaatgcg ccgctacagg gcgcgtc