pCMV-SPORT1

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCMV-SPORT1

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCMV-SPORT1

载体基本信息

出品公司:	Ybscience
载体名称:	pCMV-SPORT1
质粒类型:	哺乳动物表达载体；cDNA克隆载体；I.M.A.G.E.载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	CMV
载体大小:	4380 bp
5' 测序引物及序列:	CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列:	SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	--
克隆菌株:	DH5α 等
宿主细胞（系）:	哺乳动物细胞
备注:	用于cDNA文库的构建
稳定性:	瞬表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

载体序列

LOCUS       Exported                4380 bp ds-DNA     circular SYN 28-FEB-2013DEFINITION  Vector for cloning and transient mammalian cell expression of cDNAs.ACCESSION   .VERSION     .KEYWORDS    pCMV SPORTSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 4380)  AUTHORS   I.M.A.G.E. ConsortiumFEATURES             Location/Qualifiers     source          1..4380                     /organism="synthetic DNA construct"                     /lab_host="Mammalian Cells"                     /mol_type="other DNA"     polyA_signal    125..259                     /note="SV40 poly(A) signal"                     /note="SV40 polyadenylation signal"     promoter        646..664                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"     misc_feature    682..779                     /note="MCS"                     /note="multiple cloning site"     promoter        complement(787..805)                     /note="SP6 promoter"                     /note="promoter for bacteriophage SP6 RNA polymerase"     promoter        complement(921..1124)                     /note="CMV promoter"                     /note="human cytomegalovirus (CMV) immediate early                      promoter"     enhancer        1125..1428                     /note="CMV enhancer"                     /note="human cytomegalovirus immediate early enhancer"     protein_bind    1458..1474                     /bound_moiety="lac repressor encoded by lacI"                     /note="lac operator"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."     promoter        complement(1482..1513)                     /note="lac UV5 promoter"                     /note="E. coli lac promoter with an ""up"" mutation"     rep_origin      complement(1975..2563)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     protein_bind    2641..2674                     /bound_moiety="Cre recombinase"                     /note="loxP"                     /note="Cre-mediated recombination occurs in the 8-bp core                      sequence (GCATACAT)."     CDS             complement(2776..3636)                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /note="AmpR"                     /note="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     rep_origin      complement(3924..4379)                     /direction=LEFT                     /note="f1 ori"                     /note="f1 bacteriophage origin of replication; arrow                      indicates direction of (+) strand synthesis"ORIGIN        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcttataata cgactcacta      661 tagggagaga gctatgacgt cgcatgcacg cgtaagcttg ggcccctcga gggatcctct      721 agagcggccg ccgactagtg agctcgtcga cccgggaatt ccggaccggt acctgcaggc      781 gtaccttcta tagtgtcacc taaatagctt tttgcaaaag cctaggctag agtccggagg      841 ctggatcggt cccggtgtct tctatggagg tcaaaacagc gtggatggcg tctccaggcg      901 atctgacggt tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg      961 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg     1021 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca     1081 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg     1141 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa     1201 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac     1261 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg     1321 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg     1381 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgac ctgcacgatg     1441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acattatacg agccggaagc     1501 tataaagtgt aaagcctggg gtgcctaatg agtgaaaggg cctcgtatac gcctattttt     1561 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa     1621 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat     1681 gagacaataa ccctgataaa tgcttcaata atattgaaaa acgcgcgaat tgcaagctct     1741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc     1801 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca     1861 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg     1921 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca     1981 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     2041 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     2101 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     2161 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     2221 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     2281 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     2341 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     2401 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     2461 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     2521 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     2581 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgcc     2641 ataacttcgt atagcataca ttatacgaag ttatggcatg agattatcaa aaaggatctt     2701 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     2761 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     2821 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     2881 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     2941 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     3001 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     3061 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     3121 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     3181 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     3241 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     3301 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     3361 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     3421 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     3481 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     3541 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     3601 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     3661 aagcatttat cagggttatt gtctcatgcc aggggtgggc acacatattt gataccagcg     3721 atccctacac agcacataat tcaatgcgac ttccctctat cgcacatctt agacctttat     3781 tctccctcca gcacacatcg aagctgccga gcaagccgtt ctcaccagtc caagacctgg     3841 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac     3901 atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa aattcgcgtt     3961 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta     4021 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc     4081 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg     4141 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact     4201 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt     4261 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc     4321 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc