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保存条件 ：-20℃
保质期 ：6个月
英文名 ：pKD20
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pKD20载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pKD20 |
| 质粒类型: | 细菌表达；基因敲除系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6078 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 筛选标记: | -- |
| 备注: | -- |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS AY048745 6078 bp DNA circular SYN 11-SEP-2001DEFINITION Red recombinase plasmid pKD20, complete sequence.ACCESSION AY048745VERSION AY048745.1 GI:15554338KEYWORDS .SOURCE Red recombinase plasmid pKD20 ORGANISM Red recombinase plasmid pKD20 other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 6078) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000) PUBMED 10829079REFERENCE 2 (bases 1 to 6078) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE Direct Submission JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University, Lilly Hall of Life Sciences, West Lafayette, IN 47907, USAFEATURES Location/Qualifiers source 1..6078 /organism="Red recombinase plasmid pKD20" /mol_type="genomic DNA" /db_xref="taxon:170494" CDS complement(11..889) /codon_start=1 /transl_table=11 /product="araC protein" /protein_id="AAL02039.1" /db_xref="GI:15554339" misc_feature complement(1040..1068) /note="Pc" misc_feature 1165..1192 /note="ParaB" CDS 1244..1660 /note="recombination;138" /codon_start=1 /transl_table=11 /product="gam protein" /protein_id="AAL02040.1" /db_xref="GI:15554340" CDS 1666..2451 /note="recombination;261" /codon_start=1 /transl_table=11 /product="bet protein" /protein_id="AAL02041.1" /db_xref="GI:15554341" CDS 2448..3128 /note="exo" /codon_start=1 /transl_table=11 /product="exonuclease" /protein_id="AAL02042.1" /db_xref="GI:15554342" CDS complement(3245..4195) /codon_start=1 /transl_table=11 /product="repA101 protein" /protein_id="AAL02043.1" /db_xref="GI:15554343" rep_origin 4199..4939 /note="oriR101" CDS complement(5087..5947) /note="bla; ampicillin resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAL02044.1" /db_xref="GI:15554344"ORIGIN 1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga 1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc 1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat 1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga 1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc 1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt 1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac 1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc 1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa 1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc 1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc 1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc 1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca 1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa 2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg 2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga 2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact 2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt 2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc 2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa 2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca 2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc 2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc 2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg 2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg 2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga 2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg 2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg 2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga 2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc 3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg 3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat 3121 ggcgatgata agctagccca tgggtatgga cagttttccc tttgatatgt aacggtgaac 3181 agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa gagccataag 3241 aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt tgtttttgcg 3301 tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc aaaaattttg 3361 cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt tttcttagtc 3421 cgttacgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca ttcattttta 3481 tctggttgtt ctcaagttcg gttacgagat ccatttgtct atctagttca acttggaaaa 3541 tcaacgtatc agtcgggcgg cctcgcttat caaccaccaa tttcatattg ctgtaagtgt 3601 ttaaatcttt acttattggt ttcaaaaccc attggttaag ccttttaaac tcatggtagt 3661 tattttcaag cattaacatg aacttaaatt catcaaggct aatctctata tttgccttgt 3721 gagttttctt ttgtgttagt tcttttaata accactcata aatcctcata gagtatttgt 3781 tttcaaaaga cttaacatgt tccagattat attttatgaa tttttttaac tggaaaagat 3841 aaggcaatat ctcttcacta aaaactaatt ctaatttttc gcttgagaac ttggcatagt 3901 ttgtccactg gaaaatctca aagcctttaa ccaaaggatt cctgatttcc acagttctcg 3961 tcatcagctc tctggttgct ttagctaata caccataagc attttcccta ctgatgttca 4021 tcatctgagc gtattggtta taagtgaacg ataccgtccg ttctttcctt gtagggtttt 4081 caatcgtggg gttgagtagt gccacacagc ataaaattag cttggtttca tgctccgtta 4141 agtcatagcg actaatcgct agttcatttg ctttgaaaac aactaattca gacatacatc 4201 tcaattggtc taggtgattt taatcactat accaattgag atgggctagt caatgataat 4261 tactagtcct tttcctttga gttgtgggta tctgtaaatt ctgctagacc tttgctggaa 4321 aacttgtaaa ttctgctaga ccctctgtaa attccgctag acctttgtgt gttttttttg 4381 tttatattca agtggttata atttatagaa taaagaaaga ataaaaaaag ataaaaagaa 4441 tagatcccag ccctgtgtat aactcactac tttagtcagt tccgcagtat tacaaaagga 4501 tgtcgcaaac gctgtttgct cctctacaaa acagacctta aaaccctaaa ggcttaagta 4561 gcaccctcgc aagctcggtt gcggccgcaa tcgggcaaat cgctgaatat tccttttgtc 4621 tccgaccatc aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac 4681 ggctctggca gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag 4741 gaaactaccc ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg 4801 tctgctatgt ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc 4861 agtctgacca cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta 4921 aggcagcggt atcatcaacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 5041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 5101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 5161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 5221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 5281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 5341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 5401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 5461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 5521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 5581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 5641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 5701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 5761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 5821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 5881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 5941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 6001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 6061 cccgaaaagt gccacctg
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