pEGM-7ZF(+)

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pEGM-7ZF(+)

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pEGM-7ZF(+)

载体基本信息

出品公司:	Ybscience
载体名称:	pEGM-7ZF(+)
质粒类型:	大肠杆菌克隆载体
高拷贝/低拷贝:	--
启动子:	--
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3000bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	Ampicillin
筛选标记:	--
备注:	--
稳定性:	--
组成型:	--
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

载体简介

 

The pGEM?-7Zf(+) Vector(a) is a derivative of the pGEM?-3Zf(+) Vector(a) and containsthe origin of replication of the filamentous phage f1. The plasmid serves as astandard cloning vector, as a template for in vitro transcription, and it can be usedfor the production of circular ssDNA. The plasmid contains SP6 and T7 RNA polymerasepromoters flanking a region of multiple cloning sites within the α-peptidecoding region of β-galactosidase (1). Insertional inactivation of the α-peptide allowsrecombinant clones to be directly identified by color screening on indicator plates.The multiple cloning region is unique and includes restriction sites for Apa I, Aat II,Sph I, Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Hind III, BamH I, Sac I,BstX I and Nsi I. This arrangement is designed specifically for generation of unidirectionaldeletions with the Erase-a-Base? System(b). The polylinker containsrestriction enzyme sites that produce 5′ overhangs or blunt ends (sensitive toExonuclease III) flanked on both sides by blocks of restriction sites that generate3′ overhangs (resistant to Exonuclease III).For induction of ssDNA, bacterial cells containing pGEM?-7Zf(+) recombinants areinfected with an appropriate helper phage. The plasmid then enters the f1 replicationmode, and the resulting ssDNA is exported from the cell as an encapsidated viruslikeparticle. The sequence of the ssDNA rescued upon infection with helper phageis complementary to the sequence shown in Figure 1. The exported ssDNA can beused for mutagenesis in vitro or can be sequenced using the T7 Promoter Primer(Cat.# Q5021) or pUC/M13 Forward Primer (Cat.# Q5391, Q5601).

 

载体序列

LOCUS       pGEM-7Zf-	3000 bp 	DNA	SYNDEFINITION  pGEM-7Zf-ACCESSION   KEYWORDS    SOURCE        ORGANISM  other sequences; artificial sequences; vectors.FEATURES             Location/Qualifiers     source          1..3000                     /organism="pGEM-7Zf-"                     /mol_type="other DNA"     misc_feature    complement(123..140)                     /label="Sp6_primer"     promoter        complement(154..172)                     /label="M13_reverse_primer"     misc_feature    complement(171..193)                     /label="M13_pUC_rev_primer"     promoter        complement(207..236)                     /label="lac_promoter"     rep_origin      complement(545..1164)                     /label="pBR322_origin"     gene            complement(1319..2179)                     /label="Ampicillin"                     /gene="Ampicillin"     CDS             complement(1319..2179)                     /label="ORF frame 3"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        complement(2221..2249)                     /label="AmpR_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     rep_origin      2382..2688                     /label="f1_origin"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    complement(2832..2980)                     /label="lacZ_a"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     misc_feature    2946..2968                     /label="M13_pUC_fwd_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        2961..2977                     /label="M13_forward20_primer"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"     promoter        2984..2                     /label="T7_promoter"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW*"ORIGIN    1 gggcgaattg ggcccgacgt cgcatgctcc tctagactcg aggaattcgg taccccgggt   61 tcgaaatcga taagcttgga tccggagagc tcccaacgcg ttggatgcat agcttgagta  121 ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa  181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct  241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc  301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg  361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc  421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag  481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa  541 aggccgcgtt gctggcgttt ttcgataggc tccgcccccc tgacgagcat cacaaaaatc  601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc  661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg  721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt  781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc  841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc  901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag  961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 1021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 1081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 1141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 1201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 1261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 1321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 1381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 1441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1621 ttgttggcat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 1681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 1741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 1801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 1861 tgactggtga gtactcaacc aagtcattct gagaataccg cgcccggcga ccgagttgct 1921 cttgcccggc gtcaatacgg gataatagtg tatgacatag cagaacttta aaagtgctca 1981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 2041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 2101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 2161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 2221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 2281 cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt 2341 aaggagaaaa taccgcatca ggcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg 2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 2521 ggctcccttt agggttccga tttagagctt tacggcacct cgaccgcaaa aaacttgatt 2581 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 2701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 2761 atgagctgat ttaacaaata tttaacgcga attttaacaa aatattaacg tttacaattt 2821 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 2881 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg 2941 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata