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保存条件 ：-20℃
保质期 ：6个月
英文名 ：pKD46
库存 ：大量
供应商 ：钰博生物
规格 ：1ug/5ug
pKD46载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pKD46 |
| 质粒类型: | 细菌表达；基因敲除系统 |
| 高拷贝/低拷贝: | -- |
| 启动子: | -- |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 6329 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | -- |
| 筛选标记: | -- |
| 备注: | pKD46 is more efficient than pKD20 for making gene disruptions. |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pKD46 6329 bp DNA circular SYN 11-SEP-2001DEFINITION Red recombinase plasmid pKD46, complete sequence.ACCESSION AY048746VERSION AY048746.1 GI:15554345KEYWORDS .SOURCE Red recombinase plasmid pKD46 ORGANISM Red recombinase plasmid pKD46 other sequences; artificial sequences; vectors.REFERENCE 1 (bases 1 to 6329) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (12), 6640-6645 (2000) PUBMED 10829079REFERENCE 2 (bases 1 to 6329) AUTHORS Datsenko,K.A. and Wanner,B.L. TITLE Direct Submission JOURNAL Submitted (29-JUL-2001) Biological Sciences, Purdue University, Lilly Hall of Life Sciences, West Lafayette, IN 47907, USAFEATURES Location/Qualifiers source 1..6329 /organism="Red recombinase plasmid pKD46" /mol_type="genomic DNA" /db_xref="taxon:170495" CDS complement(11..889) /codon_start=1 /transl_table=11 /product="araC protein" /protein_id="AAL02045.1" /db_xref="GI:15554346" misc_feature complement(1040..1068) /note="Pc" misc_feature 1165..1192 /note="ParaB" CDS 1244..1660 /note="recombination;138" /codon_start=1 /transl_table=11 /product="gam protein" /protein_id="AAL02046.1" /db_xref="GI:15554347" CDS 1666..2451 /note="recombination;261" /codon_start=1 /transl_table=11 /product="bet protein" /protein_id="AAL02047.1" /db_xref="GI:15554348" CDS 2448..3128 /note="exo" /codon_start=1 /transl_table=11 /product="exonuclease" /protein_id="AAL02048.1" /db_xref="GI:15554349" misc_feature 3118..3387 /note="tL3" CDS 3125..3307 /note="60a" /codon_start=1 /transl_table=11 /product="orf60a protein" /protein_id="AAL02051.1" /db_xref="GI:15554352" CDS complement(3496..4446) /codon_start=1 /transl_table=11 /product="repA101 protein" /protein_id="AAL02049.1" /db_xref="GI:15554350" rep_origin 4450..5190 /note="oriR101" CDS complement(5338..6198) /note="bla; ampicillin resistance" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAL02050.1" /db_xref="GI:15554351"ORIGIN 1 catcgattta ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac 61 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat 121 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca 181 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct 241 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga 301 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat 361 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct 421 caagcagatt tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga 481 tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg 541 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt 601 aaacccactg gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc 661 ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca 721 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt 781 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg 841 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac 901 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg 961 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt 1021 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca 1081 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat 1141 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat 1201 acccgttttt ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga 1261 aactgagatc aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc 1321 atttcgcggg cgatattttc acagctattt caggagttca gccatgaacg cttattacat 1381 tcaggatcgt cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga 1441 gaaagaggca gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc 1501 gctatgcatc gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt 1561 tgatgacgat gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac 1621 cattgctcac caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc 1681 gcaacgctgg ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa 1741 ctgatcacca ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc 1801 gcattactga tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc 1861 tttcctgata agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc 1921 atcaatgaaa accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca 1981 tgccggattt accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa 2041 tgccgccgcg aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg 2101 catcccaaac ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga 2161 tttgctggta tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact 2221 gcagaacgtc agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt 2281 aacactctgc tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc 2341 cagatatttc gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa 2401 gctcttggat tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca 2461 ttatcctgca gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc 2521 acaaattacg gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc 2581 gctccggaaa gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg 2641 tttgcaccgg tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg 2701 agaacgacgc cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga 2761 tcatctatcg cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg 2821 gcaacggcct tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg 2881 gtggtttcga ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga 2941 cgcgaaaaaa tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc 3001 attatgtcgt gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg 3061 agttcatcga aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat 3121 ggcgatgacg catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt 3181 tacaaagcga ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca 3241 gcggctggct gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt 3301 gagttaagaa cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg 3361 ccaataccag tagaaacaga cgaagaatcc atgggtatgg acagttttcc ctttgatatg 3421 taacggtgaa cagttgttct acttttgttt gttagtcttg atgcttcact gatagataca 3481 agagccataa gaacctcaga tccttccgta tttagccagt atgttctcta gtgtggttcg 3541 ttgtttttgc gtgagccatg agaacgaacc attgagatca tacttacttt gcatgtcact 3601 caaaaatttt gcctcaaaac tggtgagctg aatttttgca gttaaagcat cgtgtagtgt 3661 ttttcttagt ccgttacgta ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc 3721 attcattttt atctggttgt tctcaagttc ggttacgaga tccatttgtc tatctagttc 3781 aacttggaaa atcaacgtat cagtcgggcg gcctcgctta tcaaccacca atttcatatt 3841 gctgtaagtg tttaaatctt tacttattgg tttcaaaacc cattggttaa gccttttaaa 3901 ctcatggtag ttattttcaa gcattaacat gaacttaaat tcatcaaggc taatctctat 3961 atttgccttg tgagttttct tttgtgttag ttcttttaat aaccactcat aaatcctcat 4021 agagtatttg ttttcaaaag acttaacatg ttccagatta tattttatga atttttttaa 4081 ctggaaaaga taaggcaata tctcttcact aaaaactaat tctaattttt cgcttgagaa 4141 cttggcatag tttgtccact ggaaaatctc aaagccttta accaaaggat tcctgatttc 4201 cacagttctc gtcatcagct ctctggttgc tttagctaat acaccataag cattttccct 4261 actgatgttc atcatctgag cgtattggtt ataagtgaac gataccgtcc gttctttcct 4321 tgtagggttt tcaatcgtgg ggttgagtag tgccacacag cataaaatta gcttggtttc 4381 atgctccgtt aagtcatagc gactaatcgc tagttcattt gctttgaaaa caactaattc 4441 agacatacat ctcaattggt ctaggtgatt ttaatcacta taccaattga gatgggctag 4501 tcaatgataa ttactagtcc ttttcctttg agttgtgggt atctgtaaat tctgctagac 4561 ctttgctgga aaacttgtaa attctgctag accctctgta aattccgcta gacctttgtg 4621 tgtttttttt gtttatattc aagtggttat aatttataga ataaagaaag aataaaaaaa 4681 gataaaaaga atagatccca gccctgtgta taactcacta ctttagtcag ttccgcagta 4741 ttacaaaagg atgtcgcaaa cgctgtttgc tcctctacaa aacagacctt aaaaccctaa 4801 aggcttaagt agcaccctcg caagctcggt tgcggccgca atcgggcaaa tcgctgaata 4861 ttccttttgt ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc 4921 gctgcgctca cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg 4981 attcatgcaa ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt 5041 tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc 5101 tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa 5161 tgcacccagt aaggcagcgg tatcatcaac ggggtctgac gctcagtgga acgaaaactc 5221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 5521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 5581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 5641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 5701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 5761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 5821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 5881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 5941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6301 gcgcacattt ccccgaaaag tgccacctg//
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