单疱疹酶联免疫试剂盒（美国FOCUS）

美国FOCUS单疱疹试剂盒

广州健仑生物公司提供以下单疱疹试剂盒：

ELISA IgG  单疱疹1酶免ELISA试剂盒FOCUS  96T

ELISA IgG  单疱疹2酶免ELISA试剂盒 FOCUS  96T

Immunoblot IgG 单疱疹1/2型免疫印迹法 24份/盒

TEST PROCEDURE
1.  Bring all reagents to room temperature before use. Remove the Antigen Well packet from cold storage. To avoid condensation, allow micro-well strips to
reach room temperature before opening the foil packet. If less than a full plate is to be used, return unused strips to the foil packet with desiccant and reseal
completely. Store unused antigen wells at 2 to 8°C. (Note: At the end of the assay, retain the frame for use with the remaining strips.)
2.  OPTIONAL 5 minute Pre-Soak. If omitting pre-soak, proceed to Step 3.
Fill wells with 1X Wash Buffer and soak for 5 minutes. Decant (or aspirate) the Antigen Wells and tap vigorously to remove 1X Wash Buffer. Blot the
emptied Antigen Wells face down on clean paper towels or absorbent paper to remove residual 1X Wash Buffer.
3.  Dispense 100 µL of the Sample Diluent into the “blank” well and 100 µL of each diluted specimen, control or calibrator (see Specimen, Controls, and
Calibrator Preparation, above) into the appropriate wells. (Note: For runs with more than 48 wells it is recommended that 250 µL of each diluted sample
first be added to a blank microtiter plate in the location corresponding to that in the ELISA wells. The samples can then be efficiently transferred into the
Antigen Wells with a 100 µL 8 or 12-channel pipettor.)
4.  Cover plates with sealing tape (or place in a humid chamber), and incubate for 60 ± 1 minute at room temperature (20 to 25°C).
5.  Remove sealing tape (or remove wells from the humid chamber), and empty the contents of the wells into a sink or a discard basin.
6.  Fill each well with a gentle stream of 1X Wash Buffer solution from a wash bottle then empty contents into a sink or a discard basin.
7.  Repeat wash (step 6) an additional 2 times.
8.  Tap the antigen wells vigorously to remove 1X Wash Buffer. Blot the emptied Antigen Wells face down on clean paper towels or absorbent paper to
remove residual 1X Wash Buffer.
9.  Dispense 100 µL Conjugate to all wells, using a 100 µL 8 or 12-channel pipettor.
10. Cover plates with sealing tape (or place in a humid chamber) and incubate for 30 ± 1 minutes at room temperature (20 to 25°C).
11. Repeat wash steps 5 through 8.
12. Pipet 100 µL of Substrate Reagent to all wells, using a 100 µL 8 or 12-channel pipettor. Begin incubation timing with the addition of Substrate Reagent to
the first well. (Note: Never pour the substrate reagent into the same trough as was used for the conjugate.)
13. Incubate for 10 ± 1 minutes at room temperature (20 to 25°C).
14. Stop the reaction by adding 100 µL of Stop Reagent to all wells using a 100 µL 8 or 12-channel pipettor. Add the Stop Reagent in the same sequence and at
the same pace as the Substrate was added. In antibody-positive wells, color should change from blue to yellow.
15. Gently blot the outside bottom of wells with a paper towel to remove droplets that may interfere with reading by the spectrophotometer. Do not rub with the
paper towel as it may scratch the optical surface of the well. (Note: Large bubbles on the surface of the liquid may affect the OD readings.)
16. Measure the absorbance of each well within 1 hour of stopping the assay. Set the microwell spectrophotometer at a wavelength of 450 nm. Zero the
instrument on the blank wells, or correct all ODs by manually subtracting the blank ODs.

单疱疹1/2型免疫印迹法试剂盒（美国FOCUS）

单纯疱疹是一种由单纯疱疹病毒所致的病毒性皮肤病，中医称为热疮。能引起人类多种疾病，如龈口炎（gingivostomatitis）、角膜结膜炎（keratoconjunctivitis）、脑炎（encephalitis）以及生殖系统感染和新生儿的感染。

 

单疱疹1/2型免疫印迹法试剂盒（美国FOCUS）

更多详细说明及原版英文说明书请联系工作人员。

【公司名称】 广州健仑生物科技有限公司
【联系电话】 13802525278 020-82574011  杨永汉 
【公司传真】 020-32206070
【腾讯 QQ 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室