pET101/D-TOPO

pET101/D-TOPO

价格: ¥800 - 1200

品牌:Ybscience

货号:YB-0028

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保存条件 :-20℃

保质期 :6个月

英文名 :pET101/D-TOPO

库存 :大量

供应商 :钰博生物

规格 :1ug/5ug

pET101/D-TOPO

pET101载体基本信息

出品公司: Ybscience
载体名称: pET101 ; pET101/D-TOPO
质粒类型: 大肠杆菌表达载体;细菌表达载体;TOPO载体
高拷贝/低拷贝: 低拷贝
克隆方法: TOPO
启动子: T7
载体大小: 5753 bp
5' 测序引物及序列: T7 promoter 5’TAATACGACTCACTATAGGG 3’
3' 测序引物及序列: T7 terminator 5’GCTAGTTATTGCTCAGCGG 3’
载体标签: V5 tag、6xHis tag (C-term)
载体抗性: 氨苄青霉素
克隆菌株: TOP10
表达菌株:
BL21-Star(DE3) 、BL21-Star(DE3) pLysS
 
备注:
pET101含有T7聚合酶结合位点和RBS核糖体结合位点,可以做体外转录和翻译。
 
组成型/诱导型: 诱导型 (IPTG)
病毒/非病毒: 非病毒
 

pET101载体质粒图谱和多克隆位点信息

pET101载体图谱



pET101多克隆位点

pET101载体特征



pET101载体特征

pET101载体简介

 

pET101载体序列

LOCUS       pET101-D-TOPO               5753 bp ds-DNA     circular SYN 15-AUG-2016DEFINITION  synthetic circular DNAKEYWORDS    pET101-D-TOPOSOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 5753)  AUTHORS   Biofeng Lab.FEATURES             Location/Qualifiers     source          1..5753                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     promoter        209..227                     /note="T7 promoter"                     /note="promoter for bacteriophage T7 RNA polymerase"     protein_bind    228..252                     /bound_moiety="lac repressor encoded by lacI"                     /note="lac operator"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."     misc_feature    282..296                     /note="RBS"     misc_feature    282..288                     /note="RBS"     misc_feature    293..296                     /note="RBS"     misc_feature    297..310                     /note="TOPO site"     CDS             333..374                     /codon_start=1                     /product="epitope tag from simian virus 5"                     /note="V5 tag"                     /translation="GKPIPNPLLGLDST"     CDS             384..401                     /codon_start=1                     /product="6xHis affinity tag"                     /note="6xHis"                     /translation="HHHHHH"     terminator      416..544                     /note="T7 terminator"                     /note="transcription terminator for bacteriophage T7 RNA                      polymerase"     promoter        complement(694..722)                     /note="tet promoter"                     /note="E. coli promoter for tetracycline efflux protein                      gene"     promoter        845..943                     /gene="bla"                     /note="bla promoter"     CDS             944..1804                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /note="AmpR"                     /note="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     rep_origin      1949..2622                     /direction=RIGHT                     /note="pBR322"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"     misc_feature    2749..2888                     /note="bom"                     /note="basis of mobility region from pBR322"     CDS             complement(2990..3181)                     /codon_start=1                     /gene="rop"                     /product="Rop protein, which maintains plasmids at low copy                     number"                     /note="rop"                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA                     DELYRSCLARFGDDGENL"     CDS             complement(4493..5584)                     /codon_start=1                     /gene="lacI"                     /product="lac repressor"                     /note="lacI"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."                     /translation="MVNVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAM                     AELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVER                     SGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGD                     WSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTED                     SSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTA                     SPRALADSLMQLARQVSRLESGQ"     promoter        complement(5576..5653)                     /gene="lacI"                     /note="lacI promoter"ORIGIN        1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa       61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat      121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta      181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg      241 gataacaatt cccctctaga aataattttg tttaacttta agaaggaatt caggagccct      301 tcaccaaggg cgagctcaat tcgaagcttg aaggtaagcc tatccctaac cctctcctcg      361 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttga tccggctgct      421 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa      481 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc      541 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca      601 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac      661 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc      721 aaacatgaga attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt      781 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg      841 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca      901 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt      961 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga     1021 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga     1081 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat     1141 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca     1201 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt     1261 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac     1321 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct     1381 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga     1441 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac     1501 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat     1561 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg     1621 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc     1681 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc     1741 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg     1801 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta     1861 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg     1921 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga     1981 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt     2041 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag     2101 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa     2161 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag     2221 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca     2281 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac     2341 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa     2401 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc     2461 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg     2521 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc     2581 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc     2641 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag     2701 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta     2761 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc     2821 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca     2881 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc     2941 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt     3001 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa     3061 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg     3121 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca     3181 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag     3241 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg     3301 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat     3361 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga     3421 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa     3481 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt     3541 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc     3601 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa     3661 cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct     3721 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg     3781 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct     3841 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca     3901 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc     3961 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc     4021 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc     4081 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta     4141 gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt     4201 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga     4261 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc     4321 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat     4381 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg     4441 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc     4501 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg     4561 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca     4621 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg     4681 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc     4741 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact     4801 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg     4861 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt     4921 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag     4981 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt     5041 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa     5101 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg     5161 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga     5221 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta     5281 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa     5341 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt     5401 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt     5461 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg     5521 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca     5581 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc     5641 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag     5701 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atg//

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