pCS105

保存条件 ：-20℃

保质期 ：6个月

英文名 ：pCS105

库存 ：大量

供应商 ：钰博生物

规格 ：1ug/5ug

pCS105

载体基本信息

出品公司:	Ybscience
载体名称:	pCS105
质粒类型:	细菌表达载体；双表达载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	SP6，T3
载体大小:	4131 bp
5' 测序引物及序列:	SP6 Forward: ATTTAGGTGACACTATAG
3' 测序引物及序列:	M13 Reverse: CAGGAAACAGCTATGAC
载体标签:	--
载体抗性:	氨苄青霉素
表达菌株:	原核细胞
备注:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

pCS105载体质粒图谱和多克隆位点信息

 

载体简介

 

pCS105载体序列

LOCUS       Exported File           4131 bp ds-DNA    circular SYN 11-七月-2016DEFINITION  Vector that allows high-level transient expression in vertebrate             cells as well as?in vitro transcription/translation.ACCESSION   DQ649431VERSION     .KEYWORDS    pCS105SOURCE      synthetic DNA construct  ORGANISM  synthetic DNA constructREFERENCE   1  (bases 1 to 4131)  AUTHORS   .  TITLE     Direct Submission  JOURNAL   Exported 2016-07-11 from SnapGene Viewer 1.5.3            http://www.snapgene.comFEATURES             Location/Qualifiers     source          1..4131                     /organism="synthetic DNA construct"                     /mol_type="other DNA"     promoter        35..53                     /note="SP6 promoter"                     /note="promoter for bacteriophage SP6 RNA polymerase"                     /note="color: #ffffff; direction: RIGHT"     misc_feature    79..148                     /note="MCS1"                     /note="color: #ffff00"     misc_feature    145..172                     /note="stop codons"                     /note="stop codons in all three reading frames"                     /note="color: #ff0000"     polyA_signal    172..306                     /note="SV40 poly(A) signal"                     /note="SV40 polyadenylation signal"                     /note="color: #a6acb3"     misc_feature    375..410                     /note="MCS2"                     /note="color: #00ffff"     promoter        complement(423..441)                     /note="T3 promoter"                     /note="promoter for bacteriophage T3 RNA polymerase"                     /note="color: #ffffff; direction: LEFT"     primer_bind     complement(462..478)                     /note="M13 rev"                     /note="common sequencing primer, one of multiple similar                      variants"                     /note="color: #a020f0; direction: LEFT"     protein_bind    486..502                     /bound_moiety="lac repressor encoded by lacI"                     /note="lac operator"                     /note="The lac repressor binds to the lac operator to                      inhibit transcription in E. coli. This inhibition can be                      relieved by adding lactose or                      isopropyl-beta-D-thiogalactopyranoside (IPTG)."                     /note="color: #31849b"     promoter        complement(510..540)                     /note="lac promoter"                     /note="promoter for the E. coli lac operon"                     /note="This reverse directional feature has 3 segments:                     ???1:?510?..?516?/?#ffffff?/?-10                     ???2:?517?..?534?/?#ffffff                     ???3:?535?..?540?/?#ffffff?/?-35"     rep_origin      complement(864..1452)                     /direction=LEFT                     /note="ori"                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of                      replication"                     /note="color: #ffff00"     CDS             complement(1623..2483)                     /codon_start=1                     /gene="bla"                     /product="beta-lactamase"                     /note="AmpR"                     /note="confers resistance to ampicillin, carbenicillin, and                     related antibiotics"                     /note="This feature has 2 segments:                     ???1:?1623?..?2414?/?#ccffcc                     ???2:?2415?..?2483?/?#ccffcc?/?signal?sequence                     Cleavage site after base 2414"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS                     LIKHW"     promoter        complement(2484..2588)                     /gene="bla"                     /note="AmpR promoter"                     /note="color: #ffffff; direction: LEFT"     rep_origin      complement(2614..3069)                     /direction=LEFT                     /note="f1 ori"                     /note="f1 bacteriophage origin of replication; arrow                      indicates direction of (+) strand synthesis"                     /note="color: #ffff00"     promoter        3147..4131                     /note="CMV IE94 promoter"                     /note="enhancer/promoter region of simian cytomegalovirus                      major immediate early transcription unit IE94"                     /note="color: #ffffff; direction: RIGHT"ORIGIN        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg      121 cggccgcaag gcctctcgag cctctagaac tatagtgagt cgtattacgt agatccagac      181 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc      241 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa      301 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag      361 gttttttaat tcgcggcgcg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt      421 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg      481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa      541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct      601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga      661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc      721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa      781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt      841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa      901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt      961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg     1021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc     1081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc     1141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta     1201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct     1261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc     1321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa     1381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa     1441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa     1501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt     1561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac     1621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc     1681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc     1741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata     1801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc     1861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc     1921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca     1981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa     2041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca     2101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt     2161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt     2221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg     2281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga     2341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc     2401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg     2461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag     2521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg     2581 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa     2641 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca     2701 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga     2761 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc     2821 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc     2881 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc     2941 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg     3001 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac     3061 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg     3121 cctcttcgct attacgccag tcgatcgacc atagccaatt caatatggcg tatatggact     3181 catgccaatt caatatggtg gatctggacc tgtgccaatt caatatggcg tatatggact     3241 cgtgccaatt caatatggtg gatctggacc ccagccaatt caatatggcg gacttggcac     3301 catgccaatt caatatggcg gacttggcac tgtgccaact ggggaggggt ctacttggca     3361 cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag tccgccatat tgaattggca     3421 tggtgccaat aatggcggcc atattggcta tatgccagga tcaatatata ggcaatatcc     3481 aatatggccc tatgccaata tggctattgg ccaggttcaa tactatgtat tggccctatg     3541 ccatatagta ttccatatat gggttttcct attgacgtag atagcccctc ccaatgggcg     3601 gtcccatata ccatatatgg ggcttcctaa taccgcccat agccactccc ccattgacgt     3661 caatggtctc tatatatggt ctttcctatt gacgtcatat gggcggtcct attgacgtat     3721 atggcgcctc ccccattgac gtcaattacg gtaaatggcc cgcctggctc aatgcccatt     3781 gacgtcaata ggaccaccca ccattgacgt caatgggatg gctcattgcc cattcatatc     3841 cgttctcacg ccccctattg acgtcaatga cggtaaatgg cccacttggc agtacatcaa     3901 tatctattaa tagtaacttg gcaagtacat tactattgga agtacgccag ggtacattgg     3961 cagtactccc attgacgtca atggcggtaa atggcccgcg atggctgcca agtacatccc     4021 cattgacgtc aatggggagg ggcaatgacg caaatgggcg ttccattgac gtaaatgggc     4081 ggtaggcgtg cctaatggga ggtctatata agcaatgctc gtttagggaa c//