pMAL-p5x

pMAL-p5x

价格: ¥800 - 1200

品牌:Ybscience

货号:YB-0015

收藏

产品详情

相关推荐

保存条件 :-20℃

保质期 :6个月

英文名 :pMAL-p5x

库存 :大量

供应商 :钰博生物

规格 :1ug/5ug

pMAL-p5x
载体基本信息
出品公司: Ybscience
载体名称: pMAL-p5x
质粒类型: 大肠杆菌表达载体;蛋白表达与纯化
高拷贝/低拷贝: 低拷贝
启动子: tac
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5752bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: MBP tag
载体抗性: Ampicillin
筛选标记: --
备注: --
稳定性: --
组成型: --
病毒/非病毒: --
 

pMAL-p5x载体质粒图谱和多克隆位点信息

pMAL-p5x载体图谱



pMAL-p5x多克隆位点

pMAL-p5x载体特征

载体简介

 

The vector pMAL-p5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa.MBP fusions made with this vector include an N-terminal signal sequence, so the fusion protein is directed to the periplasm. The MBP has been engineered for tighter binding to amylose resin.A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein).The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.

 

pMAL-p5x载体序列

LOCUS       pMAL-p5X                5752 bp    DNA     circular     10-NOV-2008DEFINITION  Cloning vector pMAL-p5X, complete sequence.ACCESSIONVERSIONKEYWORDS    .SOURCE      Cloning vector pMAL-p5X  ORGANISM  Cloning vector pMAL-p5X            other sequences; artificial sequences; vectors.REFERENCE   1  (bases 1 to 5752)  AUTHORS   Riggs,P.  TITLE     Expression and purification of maltose-binding protein fusions  JOURNAL   (in) Asubel,F.A., Brent,R., Kingston,R.E., Moore,D.D.,            Seidman,J.G., Smith,J.A. and Struhl,K. (Eds.);            CURRENT PROTOCOLS IN MOLECULAR BIOLOGY: 1-14;            Greene Publishing and Wiley Interscience, New York, NY (1992)REFERENCE   2  (bases 1 to 5752)  AUTHORS   Riggs,P.D.  TITLE     Direct Submission  JOURNAL   Submitted (10-NOV-2008) Research Department, New England Biolabs,            240 County Road, Ipswich, MA 01938, USAFEATURES             Location/Qualifiers     source          1..5752                     /organism="Cloning vector pMAL-p5X"                     /mol_type="other DNA"     gene            15..1163                     /gene="lacIq"     -35_signal      15..19                     /gene="lacIq"                     /note="lacIq promoter (clockwise); TGGTG"     -10_signal      40..46                     /gene="lacIq"                     /note="lacIq promoter (clockwise); CATGATA"     CDS             81..1163                     /gene="lacIq"                     /codon_start=1                     /transl_table=11                     /product="lac repressor (LacI)"                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT                     QTASPRALADSLMQLARQVSRLESGQ"     gene            1406..3043                     /gene="malE expression cassette"     -35_signal      1406..1411                     /gene="malE expression cassette"                     /note="Ptac promoter (clockwise); TTGACA"     -10_signal      1428..1433                     /gene="malE expression cassette"                     /note="Ptac promoter (clockwise); TATAAT"     CDS             1528..2829                     /gene="malE expression cassette"                     /note="malE sequence 1528..2703; spacer and factor Xa site                     2704..2762; multiple cloning site region 2763..2829"                     /codon_start=1                     /product="maltose binding protein (MBP) fusion"                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE                     EELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT                     NSSSNNNNNNNNNNLGIEGRISHMSMGGRDIVDGSEFPAGN"     primer_bind     2662..2685                     /note="malE primer sequence (NEB #S1237S)"     misc_feature    2770..2822                     /gene="malE expression cassette"                     /note="multiple cloning site (NdeI-SbfI)"     terminator      2841..2884                     /gene="malE expression cassette"                     /note="rrnB T1 transcription terminator"     primer_bind     complement(2898..2921)                     /note="MCS reverse primer sequence (NEB #SxxxxS)"     terminator      3016..3043                     /gene="malE expression cassette"                     /note="rrnB T2 transcription terminator"     gene            3092..4022                     /gene="bla"     -35_signal      3092..3097                     /gene="bla"                     /note="bla promoter (clockwise); TTCAAA"     -10_signal      3115..3120                     /gene="bla"                     /note="bla promoter (clockwise); GACAAT"     CDS             3162..4022                     /gene="bla"                     /note="ampR (confers resistance to ampicillin)"                     /codon_start=1                     /product="beta-lactamase"                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRHIHYSQNDLVE                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA                     EIGASLIKHW"     rep_origin      4110..4698                     /note="pMB1 origin of replication (clockwise) (RNAII -35                     to RNA/DNA switch point)"     gene            complement(5068..5259)                     /gene="rop"     CDS             complement(5068..5259)                     /gene="rop"                     /codon_start=1                     /transl_table=11                     /product="ROP protein"                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH                     ADELYRSCLARFGDDGENL"BASE COUNT     1439 a   1453 c   1536 g   1324 tORIGIN        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga     1501 gcaattgacc aacaaggacc atagattatg aaaataaaaa caggtgcacg catcctcgca     1561 ttatccgcat taacgacgat gatgttttcc gcctcggctc tcgccaaaat cgaagaaggt     1621 aaactggtaa tctggattaa cggcgataaa ggctataacg gtctcgctga agtcggtaag     1681 aaattcgaga aagataccgg aattaaagtc accgttgagc atccggataa actggaagag     1741 aaattcccac aggttgcggc aactggcgat ggccctgaca ttatcttctg ggcacacgac     1801 cgctttggtg gctacgctca atctggcctg ttggctgaaa tcaccccgga caaagcgttc     1861 caggacaagc tgtatccgtt tacctgggat gccgtacgtt acaacggcaa gctgattgct     1921 tacccgatcg ctgttgaagc gttatcgctg atttataaca aagatctgct gccgaacccg     1981 ccaaaaacct gggaagagat cccggcgctg gataaagaac tgaaagcgaa aggtaagagc     2041 gcgctgatgt tcaacctgca agaaccgtac ttcacctggc cgctgattgc tgctgacggg     2101 ggttatgcgt tcaagtatga aaacggcaag tacgacatta aagacgtggg cgtggataac     2161 gctggcgcga aagcgggtct gaccttcctg gttgacctga ttaaaaacaa acacatgaat     2221 gcagacaccg attactccat cgcagaagct gcctttaata aaggcgaaac agcgatgacc     2281 atcaacggcc cgtgggcatg gtccaacatc gacaccagca aagtgaatta tggtgtaacg     2341 gtactgccga ccttcaaggg tcaaccatcc aaaccgttcg ttggcgtgct gagcgcaggt     2401 attaacgccg ccagtccgaa caaagagctg gcaaaagagt tcctcgaaaa ctatctgctg     2461 actgatgaag gtctggaagc ggttaataaa gacaaaccgc tgggtgccgt agcgctgaag     2521 tcttacgagg aagagttggt gaaagatccg cgtattgccg ccactatgga aaacgcccag     2581 aaaggtgaaa tcatgccgaa catcccgcag atgtccgctt tctggtatgc cgtgcgtact     2641 gcggtgatca acgccgccag cggtcgtcag actgtcgatg aagccctgaa agacgcgcag     2701 actaattcga gctcgaacaa caacaacaat aacaataaca acaacctcgg gatcgaggga     2761 aggatttcac atatgtccat gggcggccgc gatatcgtcg acggatccga attccctgca     2821 ggtaattaaa taagcttcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt     2881 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt     2941 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca     3001 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc     3061 tttcggtccg ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat     3121 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc     3181 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     3241 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     3301 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt ttcccaatga     3361 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag     3421 agcaactcgg tcgccacata cactattctc agaatgactt ggttgagtac tcaccagtca     3481 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     3541 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     3601 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     3661 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa     3721 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag     3781 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct     3841 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac     3901 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa     3961 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt     4021 aactgtcaga ccaagtttac tcatatatac tttagattga tttccttagg actgagcgtc     4081 aaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc     4141 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta     4201 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt     4261 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc     4321 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg     4381 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg     4441 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag     4501 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc     4561 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat     4621 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg     4681 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc     4741 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt     4801 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca     4861 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt     4921 atttcacacc gcatataagg tgcactgtga ctgggtcatg gctgcgcccc gacacccgcc     4981 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc     5041 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc     5101 gaggcagctg cggtaaagct catcagcgtg gtcgtgcagc gattcacaga tgtctgcctg     5161 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa     5221 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg     5281 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt     5341 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg     5401 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga     5461 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt     5521 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca     5581 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat     5641 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga     5701 caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaaa tt//

上海钰博生物科技有限公司

品牌商

实名认证

金牌会员

入驻年限:12年

联系人:陈圆圆

地址:上海

电话联系

购买咨询

换一个