双链DNA(dsDNA)荧光定量检测试剂盒(dsDNA HS Assay Kit)价格_品牌:翌圣生物（Yeasen）-丁香通官网

保存条件：2-8℃避光保存12个月

保质期：有效期1年

英文名：dsDNA HS Assay Kit

库存：大量

供应商：翌圣生物科技(上海)股份有限公司

CAS号：无

规格：100T

qubit试剂搭配酶标仪操作流程

dsDNA HS Assay Kit是一种快速、灵敏、精确的双链DNA（dsDNA）荧光定量检测试剂盒。本试剂盒对dsDNA具有高度选择性，在0.2-100 ng区间具有很好的线性关系。本试剂盒操作简单方便，使用时只需将检测工作液与待测dsDNA样品混合，即可使用Qubit荧光仪或荧光酶标仪进行读数。操作简单，结果可靠，是NGS大规模DNA样品定量（如Input DNA定量、DNA文库定量等）的理想选择。本试剂盒对常规的污染物如蛋白质、盐类等具有较好的耐受性。

产品组分

编号	组分名称	浓度	12640ES60	12640ES76
12640-A	dsDNA Reagent	200×concentrate in DMSO	250 μL	1.25 mL
12640-B	dsDNA Buffer	Not applicable	50 mL	250 mL
12640-C	dsDNA Standard 1	0 ng/μL in TE buffer	1 mL	5×1 mL
12640-D	dsDNA Standard 2	10 ng/μL in TE buffer	1 mL	5×1 mL

运输和保存
冰袋运输，2-8℃避光保存12个月。

注意事项
1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭；
2）对于DNA标准品，每次使用前先上下颠倒数次后瞬时离心数秒（请勿涡旋振荡）；
3）为了您的安全和健康，请穿实验服并戴一次性手套操作。
4）本产品仅用作科研用途！

实验步骤
一、使用Qubit荧光仪进行dsDNA定量检测分析

污染物对dsDNA定量检测试剂盒检测结果的影响

污染物	10 µL样品中的浓度	样品检测时浓度	检测结果
Salts
Ammonium acetate	200 mM	10 mM	OK
Sodium acetate	200 mM	10 mM	OK
Sodium chloride	200 mM	10 mM	OK
Magnesium chloride	40 mM	2 mM	OK
Organic Solvents
Phenol	2%	0.1%	OK
Ethanol	20%	1%	OK
Chloroform	4%	0.2%	OK
Detergents
Sodium dodecyl sulfate	0.2%	0.01%	OK
Triton X-100	0.02%	0.001%	OK
Other Compounds
Bovine serum albumin	400 μg/mL	20 μg/mL	OK
RNA	1×*	1×*	OK
dNTPs	2 mM	100 μM	OK
Polyethylene glycol	20%	1%	OK
Agarose	2%	0.1%	OK

*1×：表示含有与dsDNA相同浓度的RNA。

HB221117

该产品被引用文献

[1] Duan XZ, Sun JT, Wang LT, et al. Recent infection by Wolbachia alters microbial communities in wild Laodelphax striatellus populations. Microbiome. 2020;8(1):104. Published 2020 Jul 2. doi:10.1186/s40168-020-00878-x(IF:11.607)

[2] Zhang Y, An C, Zhang Y, et al. Microfluidic-templating alginate microgels crosslinked by different metal ions as engineered microenvironment to regulate stem cell behavior for osteogenesis. Mater Sci Eng C Mater Biol Appl. 2021;131:112497. doi:10.1016/j.msec.2021.112497(IF:7.328)

[3] An C, Liu W, Zhang Y, et al. Continuous microfluidic encapsulation of single mesenchymal stem cells using alginate microgels as injectable fillers for bone regeneration. Acta Biomater. 2020;111:181-196. doi:10.1016/j.actbio.2020.05.024(IF:7.242)

[4] An C, Zhang Y, Li H, et al. Thermo-responsive fluorinated surfactant for on-demand demulsification of microfluidic droplets. Lab Chip. 2021;21(18):3412-3419. Published 2021 Sep 14. doi:10.1039/d1lc00450f(IF:6.799)

[5] Gu L, Ren F, Fang X, Yuan L, Liu G, Wang S. Exosomal MicroRNA-181a Derived From Mesenchymal Stem Cells Improves Gut Microbiota Composition, Barrier Function, and Inflammatory Status in an Experimental Colitis Model. Front Med (Lausanne). 2021;8:660614. Published 2021 Jun 24. doi:10.3389/fmed.2021.660614(IF:5.093)

[6] Zhao H, Tang X, Wu M, et al. Transcriptome Characterization of Short Distance Transport Stress in Beef Cattle Blood. Front Genet. 2021;12:616388. Published 2021 Feb 10. doi:10.3389/fgene.2021.616388(IF:4.599)

[7] Gao ZR, Liu Q, Zhao J, et al. A comprehensive analysis of the circRNA-miRNA-mRNA network in osteocyte-like cell associated with Mycobacterium leprae infection. PLoS Negl Trop Dis. 2022;16(5):e0010379. Published 2022 May 2. doi:10.1371/journal.pntd.0010379(IF:4.411)

[8] Li J, Li X, Li M, et al. Differential early diagnosis of benign versus malignant lung cancer using systematic pathway flux analysis of peripheral blood leukocytes. Sci Rep. 2022;12(1):5070. Published 2022 Mar 24. doi:10.1038/s41598-022-08890-x(IF:4.380)

[9] Liang H, Zhang X, Ma Z, et al. Association of CYP3A5 Gene Polymorphisms and Amlodipine-Induced Peripheral Edema in Chinese Han Patients with Essential Hypertension. Pharmgenomics Pers Med. 2021;14:189-197. Published 2021 Feb 2. doi:10.2147/PGPM.S291277(IF:3.912)

[10] Bing XL, Zhao DS, Peng CW, Huang HJ, Hong XY. Similarities and spatial variations of bacterial and fungal communities in field rice planthopper (Hemiptera: Delphacidae) populations. Insect Sci. 2020;27(5):947-963. doi:10.1111/1744-7917.12782(IF:2.791)

[11] Zhang Y, Dai J, Yan L, Sun Y. Intra-articular injection of decellularized extracellular matrices in the treatment of osteoarthritis in rabbits. PeerJ. 2020;8:e8972. Published 2020 Apr 22. doi:10.7717/peerj.8972(IF:2.379)