Recombinant Human sCD4

英文名 ：Recombinant Human sCD4

规格 ：10ug/50ug/500ug/1mg

Recombinant Human sCD4  (CS77)

 

产品说明(Description)

Recombinant Human T-cell Surface Glycoprotein CD4 is produced by our Mammalian expression system and the target gene encoding Lys26-Trp390 is expressed with a Fc tag at the C-terminus. 

Accession #: P01730

Known as: T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;CD4;Scd4

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS,1mM EDTA,PH7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

CD4 is an approximately 55?kDa type?I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T?lymphocytes. It is a standard phenotype marker for the identification of T?cell populations. Mature human CD4 consists of a 371?amino acid extracellular region containing four immunoglobulin-like domains, a 22?aa transmembrane segment, and a 40?aa cytoplasmic domain. CD4 is expressed along with CD8 on double positive T?cells during their development in the thymus. CD4 binds directly to MHC class?II molecules on antigen presenting cells (10). This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY