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英文名 :Recombinant Human CD3 ε/CD3E
规格 :10ug/50ug/500ug/1mg
Recombinant Human CD3 ε/CD3E (C578)
产品说明(Description)
Recombinant Human T-Cell Surface Glycoprotein CD3 epsilon Chain is produced by our Mammalian expression system and the target gene encoding Asp23-Asp126 is expressed with a 6His tag at the C-terminus.
Accession #: P07766
Known as: T-Cell Surface Glycoprotein CD3 Epsilon Chain; T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e; CD3E; T3E
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.
质量控制(Quality Control)
Purity: Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity: | Bioactivity-BLI: | |
Immobilized Human CD3E-His(Cat#C578) at 10 μg/ml (100 μl/well) can bind Human Anti-CD3(Cat#NC006). The ED50 of Human Anti-CD3(Cat#NC006) is 3-15 ng/ml. |
Loaded Anti-Human/Monkey CD3E mAb-Fc(Cat#NC006) on Protein A Biosensor, can bind Human CD3E-His(Cat#C578) with an affinity constant of 1.01 nM as determined in BLI assay. |
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
苏州近岸蛋白质科技股份有限公司
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入驻年限:13年