Recombinant Mouse 5'-Nucleotidase/CD73/NT5E

英文名 ：Recombinant Mouse 5'-Nucleotidase/CD73/NT5E

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse 5'-Nucleotidase/CD73/NT5E  (CI98)

产品说明(Description)

Recombinant Mouse 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp29-Phe550 is expressed with a 6His tag at the C-terminus. 

Accession #: Q61503

Known as: 5'-nucleotidase;Ecto-5'-nucleotidase;CD73;5'-NT

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 120mM NaCl,4mM CaCl2, 20% Glycerol, pH 7.5.

 

质量控制(Quality Control) 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:  Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.
                       The specific activity is 20000-80000 pmol/min/µg.

 

Bioactivity-Elisa:		Bioactivity-BLI:
Immobilized Mouse CD73-His(Cat#CI98) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#NC034). The ED50 of Anti-Human CD73 mAb(Cat#NC034) is 12.13 ng/ml.	000000	Loaded Anti-Human CD73 mAb-Fc(Cat#NC034) on Protein A Biosensor, can bind  Mouse CD73-His(Cat#CI98) with an affinity constant of 0.31 nM as determined in BLI assay.

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.  

 

背景(Background)

Mouse CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity.CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY