Recombinant Cynomolgus LAG-3 (C-Fc)

英文名 ：Recombinant Cynomolgus LAG-3 (C-Fc)

规格 ：10ug/50μg/500μg/1mg

Recombinant Cynomolgus LAG-3 (C-Fc)  (CP99)

 

产品说明(Description)

Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Ala18-His449(Pro74) is expressed with a Fc tag at the C-terminus. 

Accession #: XP_005570011.1

Known as: Lymphocyte activation gene 3 protein;LAG3;LAG-3;Protein FDC;CD223

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, 5%Trehalose, pH 7.4.

 

质量控制(Quality Control)

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

 

Bioactivity-BLI:		Bioactivity-ELISA:
Loaded Cynomolgus LAG-3-Fc(Cat#CP99) on Protein A Biosensor, can bind Cynomolgus FGL1-His(Cat#CW69) with an affinity constant of 28.6nM as determined in BLI assay.		Immobilized Anti-Human LAG-3 mAb(Cat#NC067) at 2μg/ml (100 μl/well)can bind Cynomolgus LAG-3-Fc(Cat#CP99). The ED50 of Cynomolgus LAG-3-Fc(Cat#CP99) is 14.79 ng/ml.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY