Recombinant Mouse CD40 Ligand/TNFSF4/CD252

英文名 ：Recombinant Mouse CD40 Ligand/TNFSF4/CD252

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse CD40 Ligand/TNFSF4/CD252  (CU78)

 

产品说明(Description)

Recombinant Mouse CD40 ligand is produced by our Mammalian expression system and the target gene encoding Met112-Leu260 is expressed with a 6His tag at the N-terminus. 

Accession #: P27548

Known as: CD40 Ligand; CD40LG;HIGM1; T-B cell-activating molecule; T-BAM; TNFSF5; tumor necrosis factor (ligand) superfamily member 5; Tumor necrosis factor ligand superfamily member 5

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 200mM NaCl, 0.1mM EDTA, pH 7.0.

 

质量控制（Quality Control） 

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:   Immobilized Human CD40-Fc (Cat#CM09) at 2μg/ml (100 μl/well) can bind Human CD40LG-His(Cat#CU78).

                       The ED50 of Human CD40LG-His(Cat#C321) is 13.84ng/mL.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

CD40 Ligand, also known as TNFSF5, CD154, is a type II transmembrane glycoprotein member of the TNF superfamily. Mature mouse CD40 Ligand consists of a 22 amino acid (aa) cytoplasmic domain, a transmembrane segment, and a 214 aa extracellular region. CD40 Ligand is expressed as a homotrimer on platelets and activated T cells and B cells. It is up-regulated following stimulation of basophils, eosinophils, fibroblasts, mast cells, monocytes, natural killer cells, vascular endothelial cells, and smooth muscle cells. CD40 Ligand binds and activates CD40, which is expressed on the surface of B cells, dendritic cells,macrophages, monocytes, platelets, endothelial cells, and epithelial cells. Monomeric, dimeric, and trimeric forms of soluble CD40 Ligand bind to oligomeric CD40 on cell membranes. CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses. CD40 Ligand dysregulation on T cells and antigen presenting cells contributes to the immune deficiency associated with HIV infection and AIDS. It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY