Recombinant Human LAG-3/CD223 (C-Fc)

英文名 ：Recombinant Human LAG-3/CD223 (C-Fc)

规格 ：10ug/50ug/500ug/1mg

Recombinant Human LAG-3/CD223 (C-Fc) (CU30)

 

产品说明(Description)

Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Leu23-Leu450 is expressed with a Fc tag at the C-terminus. 

Accession #: P18627

Known as: Lymphocyte activation gene 3 protein;LAG3;LAG-3;Protein FDC;CD223

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM NaH2PO4, 150mM NaCl, 0.1M Arginine, 0.1M Glu, 10% Glycerol, 0.01% Tween20, 5% Trehalose, pH 7.4.

 

质量控制(Quality Control)

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:   Immobilized Human LAG-3-Fc(Cat#CU30) at 5μg/ml (100 μl/well) can bind Human FGL1-mFc(Cat#CW62).

                      The ED50 of Human LAG-3-Fc(Cat#CU30) is 2.1 ug/ml.

 

Bioactivity-Elisa:
Immobilized Anti-Human LAG-3 mAb(Cat#NC067) at 2μg/ml (100 μl/well)can bind Human LAG-3-Fc (Cat#CU30). The ED50 of Human LAG-3-Fc (Cat#CU30) is 6.76 ng/ml.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 

Upon receipt, store it immediately at the temperature listed below.  

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.

Store at ≤-70°C, stable for 3 months under sterile conditions after opening.

Please minimize freeze-thaw cycles.

 

背景(Background)

Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY