Recombinant Mouse TF (C-6His)

英文名 ：Recombinant Mouse TF (C-6His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse TF (C-6His)(CU70)

 

产品说明(Description)

Recombinant Mouse Tissue factor is produced by our Mammalian expression system and the target gene encoding Ala29-Glu251 is expressed with a 6His tag at the C-terminus. 

Accession #: P20352

Known as: Tissue factor; TF; Coagulation factor III; 

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,0.05%Brij35,pH7.5.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Tissue?Factor, also known as thromboplastin and CD142,?belongs to the tissue factor family which conteins 294 amino acid (aa). Mouse TF consists of a signal peptide (residues 1-28) and the mature chain (residues 29-294). It is a single-pass type I membrane protein that expressed in the liver oscillates in a circadian manner. Interacts with HSPE; the interaction, inhibited by heparin, promotes the generation of activated factor X and activates coagulation in the presence of activated factor VII. Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY