Recombinant Mouse Pleiotrophin/PTN

英文名 ：Recombinant Mouse Pleiotrophin/PTN

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse Pleiotrophin/PTN (CU58)

 

产品说明(Description)

Recombinant Mouse Pleiotrophin is produced by our Mammalian expression system and the target gene encoding Gly33-Asp168 is expressed with a 6His tag at the C-terminus. 

Accession #: P63089

Known as: Pleiotrophin; PTN; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Osteoblast-specific factor 1; OSF-1; 

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.    

 

背景（Background）

Pleiotrophin (PTN) is an secreted, strongly heparinbinding, developmentally regulated cytokine. PTN and midkine share 50% amino acid (aa) sequence identity, share some functions, and constitute a family. PTN is a highly conserved protein? human, mouse, rat, canine, porcine, equine and bovine PTN share 98% aa sequence identity or greater. During development, PTN is involved in development of brain, bone, and organs undergoing branching morphogenesis. PTN causes PTPRB dimerization and inactivates its phosphatase activity, which allows increased tyrosine phosphorylation of its substrates. Increased expression of PTN is correlated with neuronal development or stresses such as brain ischemia and Parkinson’s disease.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY