Recombinant Human LMCD1 (N, C-6His)

英文名 ：Recombinant Human LMCD1 (N, C-6His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Human LMCD1 (N, C-6His) (CG84)

产品说明(Description)

Recombinant Human LIM and cysteine-rich domains protein 1 is produced by our E.coli expression system and the target gene encoding Met1-Ser365 is expressed with a 6His tag at the N-terminus, 6His tag at the C-terminus. 

Accession #: Q9NZU5

Known as: LIM and cysteine-rich domains protein 1; LMCD1; Dyxin

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM EDTA, pH 8.0.

 

质量控制(Quality Control) 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 

Upon receipt, store it immediately at the temperature listed below.   

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt. 

Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 

Please minimize freeze-thaw cycles.

 

背景(Background)

LMCD1 is transcriptional cofactor which contains a cysteine-rich domain in the N-terminal region and 2 LIM domains in the C-terminal region. It also has several potential phosphorylation and N-myristoylation sites and a single potential N-glycosylation site. LMCD1 is expressed in many tissues with highest abundance in skeletal muscle. LMCD1 restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. It plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY