Recombinant Human KPNB1/Importin Subunit Beta-1

英文名 ：Recombinant Human KPNB1/Importin Subunit Beta-1

规格 ：10ug/50ug/500ug/1mg

Recombinant Human KPNB1/Importin Subunit Beta-1  (CP58)

 

产品说明(Description)

Recombinant Human Importin Subunit Beta-1 is produced by our E.coli expression system and the target gene encoding Met1-Ala876 is expressed with a 6His tag at the N-terminus. 

Accession #: Q14974

Known as: Importin subunit beta-1; Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97; KPNB1; NTF97       

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris,1mM DTT,30% glycerol,0.1M Nacl,pH8.0.

 

质量控制（Quality Control） 

 

Purity:            Greater than 85% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

Importin subunit beta-1(KPNB1) is a member of the importin beta family. KPNB1 contains 1 importin N-terminal domain and 19 HEAT repeats. It is involved in nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. Its functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. The import of proteins containing a classical nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits. Each of these subunits is part of the karyopherin family of proteins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. It mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5.

 

电泳(SDS-PAGE)

 

参考文献（References）

Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import[J]. Veronica Rodriguez-Bravo. et al. Cell. 2018.

 

FOR RESEARCH USE ONLY