Recombinant Human IGF2BP-2 (N-T7,C-6His)

英文名 ：Recombinant Human IGF2BP-2 (N-T7,C-6His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Human IGF2BP-2 (N-T7,C-6His) (CE98)

 

产品说明(Description)

Recombinant Human Insulin-Like Growth Factor 2 mRNA-Binding Protein 2 is produced by our E.coli expression system and the target gene encoding Met1-Thr220 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus. 

Accession #: Q9Y6M1

Known as: Insulin-Like Growth Factor 2 mRNA-Binding Protein 2; IGF2 mRNA-Binding Protein 2; IMP-2; Hepatocellular Carcinoma Autoantigen p62; IGF-II mRNA-Binding Protein 2; VICKZ Family Member 2; IGF2BP2; IMP2; VICKZ2

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS,pH 7.4.

 

质量控制（Quality Control） 

 

 

Mol Mass:  27.2kDa              AP Mol Mass: 43kDa, reducing conditions.

Purity:        Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Insulin-Like Growth Factor 2 mRNA-Binding Protein 2 (IGFBP2) belongs to the RRM IMP/VICKZ family. IGFBP2 is a cytoplasmic protein and contains four KH domains and two RRM (RNA recognition motif) domains. IGF2BP2 binds to the 5'-UTR of the Insulin-Like Growth Factor 2 (IGF2) mRNA. This binding is isoform-specific. IGF2BP2 may regulate translation of target mRNAs. Genetic variation at the IGF2BP2 gene has been associated with type 2 diabetes (T2D) by genome-wide association studies and by replication analyses.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells[J]. Fei Peng. et al. Cell Research. 2020.

Axonal localization of neuritin/CPG15 mRNA is limited by competition for HuD binding[J]. Cynthia Gomes. et al. JOURNAL OF CELL SCIENCE. 2017.

 

FOR RESEARCH USE ONLY