Recombinant Human HPD/PPD/4HPPD/HPPDase

英文名 ：Recombinant Human HPD/PPD/4HPPD/HPPDase

规格 ：10ug/50ug/500ug/1mg

Recombinant Human HPD/PPD/4HPPD/HPPDase  (CE50)

 

产品说明(Description)

Recombinant Human 4-Hydroxyphenylpyruvate Dioxygenase is produced by our E.coli expression system and the target gene encoding Met1-Met393 is expressed with a 6His tag at the N-terminus. 

Accession #: P32754

Known as: 4-Hydroxyphenylpyruvate Dioxygenase; 4-Hydroxyphenylpyruvic Acid Oxidase; 4HPPD; HPD; HPPDase; HPD; PPD

 

制剂（Formulation）

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 50mM NaCl, 1mM DTT，20% Glycerol，PH8.0. 

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 
Upon receipt, store it immediately at the temperature listed below.

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 
Please minimize freeze-thaw cycles.     

 

背景（Background）

4-Hydroxyphenylpyruvate Dioxygenase (4HPPD) belongs to the 4HPPD family. 4HPPD is a key enzyme in the degradation of tyrosine, which catalyzes the second reaction in the catabolism of tyrosine the conversation of 4-hydroxyphenylpyruvate to homogentisate. 4HPPD exists in homodimer forms, which uses zinc as a cofactor to catalyze the third step in the conversion of L-phenylalanine to fumarate and acetoacetic acid. When the active 4HPPD enzyme concentration is low in the human body, it results in high levels of tyrosine concentration in the blood, which can cause mild mental retardation at birth, and degradation in vision as a patient grows older.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY