Recombinant Human CHIT1 (C-6His)

英文名 ：Recombinant Human CHIT1 (C-6His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Human CHIT1 (C-6His) (C451)

产品说明(Description)

Recombinant Human Chitotriosidase-1 is produced by our Mammalian expression system and the target gene encoding Ala22-Asn466 is expressed with a 6His tag at the C-terminus. 

Accession #: AAI03696.1

Known as: Chitotriosidase-1; Chitinase-1; CHIT1

 

制剂(Formulation)

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 7.5.

 

质量控制(Quality Control)

 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.  

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

运输(Shipping)

The product is shipped on dry ice/polar packs. 

Upon receipt, store it immediately at the temperature listed below.  

 

保存(Storage)

Store at ≤-70°C, stable for 6 months after receipt. 

Store at ≤-70°C, stable for 3 months under sterile conditions after opening. 

Please minimize freeze-thaw cycles.

 

背景(Background)

Chitotriosidase-1 (CHIT1) is a glycoprotein that belongs to the Glycosyl Hydrolase 18 family and Chitinase class II subfamily. It is secreted by cultured macrophages and serves to degrade chitin, chitotriose and chitobiose. CHIT1 may participate in the defense against nematodes and other pathogens. It is highly expressed in the plasma of patients with Gaucher's disease type I, which can be used as diagnostic aid and to evaluate the success of treatment that brings levels back to normal. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpanzee, rat, mouse and fruit fly, respectively.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Changes in glycosylation of human blood plasma chitotriosidase in patients with type 2 diabetes. Żurawska-Płaksej E. et al. Glycoconjugate journal. 2016.

 

FOR RESEARCH USE ONLY