Recombinant Human RAGE/AGER

英文名 ：Recombinant Human RAGE/AGER

规格 ：10ug/50ug/500ug/1mg

Recombinant Human RAGE/AGER  (C423)

 

产品说明(Description)

Recombinant Human Advanced Glycosylation End Product-Specific Receptor is produced by our Mammalian expression system and the target gene encoding Ala23-Ala344 is expressed with a 6His tag at the C-terminus. 

Accession #: Q15109

Known as: Advanced Glycosylation End Product-Specific Receptor; Receptor for Advanced Glycosylation End Products; AGER; RAGE

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Advanced Glycosylation End Product-Specific Receptor (AGER) belongs to the immunoglobulin superfamily of cell surface molecules. It lies within the major histocompatibility complex (MHC) class III region on chromosome 6. Besides AGEs, AGER is also able to bind other ligands which is thought to result in pro-inflammatory gene activation. It is known that AGER serve as a mediator of both acute and chronic vascular inflammation in certain conditions such as atherosclerosis and in particular as a complication of diabetes. Furthermore, it plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes.

 

电泳(SDS-PAGE)

 

参考文献（References）

Affimers as an Alternative to Antibodies in an Affinity LC–MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Human Serum[J]. Frank Klont. et al.Journal of  Proteome Research. 2018.

Quantification of the soluble Receptor of Advanced Glycation End-Products (sRAGE) by LC-MS after enrichment by strong cation exchange (SCX) solid-phase extraction (SPE) at the protein level[J]. Frank Klont. et al. Analytica Chimica Acta. 2018.

 

FOR RESEARCH USE ONLY