Recombinant Mouse M-CSF/CSF1

英文名 ：Recombinant Mouse M-CSF/CSF1

规格 ：10ug/50ug/500ug/1mg

Recombinant Mouse M-CSF/CSF1  (CB34)

产品说明(Description)

Recombinant Mouse Macrophage colony-stimulating factor 1 is produced by our Mammalian expression system and the target gene encoding Lys33-Glu262 is expressed. 

Accession #: P07141

Known as: Macrophage colony-stimulating factor 1;CSF-1;MCSF;Csf1;Csfm

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制(Quality Control)

Purity:         Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells.

                     The ED50 for this effect is 0.04-0.2 ng/mL.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Macrophage colony-stimulating factor 1（M-csf）is a single-pass type I membrane protein . It is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity. The role of M-CSF is not only restricted to the monocyte/macrophage cell lineage. By interacting with its membrane receptor, M-CSF also modulates the proliferation of earlier hematopoietic progenitors and influence numerous physiological processes involved in immunology, metabolism, fertility and pregnancy.

 

电泳(SDS-PAGE)

 

参考文献(References)

Autophagy Promotes Cigarette Smoke-Initiated and Elastin-Driven Bronchitis-Like Airway Inflammation in Mice[J]. Hua-Qiong Huang. et al. Frontiers in Immunology. 2021.

Parathyroid Hormone Derivative with Reduced Osteoclastic Activity Promoted Bone Regeneration via Synergistic Bone Remodeling and Angiogenesis[J]. Jinghuan Huang. et al. Small. 2020.

The extract of Trachelospermum jasminoides (Lindl.) Lem. vines inhibits osteoclast differentiation through the NF-κB, MAPK and AKT signaling pathways[J]. Tao Jiang. et al. Biomedicine & Pharmacotherapy. 2020.

IGFBP7 acts as a negative regulator of RANKL-induced osteoclastogenesis and oestrogen deficiency-induced bone loss[J]. Chenyi Ye. et al. Cell Proliferation. 2019.

17β-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis[J]. Zhiheng Sun. et al. International Journal of Biological Sciences. 2021.

Histone Hyperacetylation Mediates Enhanced IL-1β Production in LPS/IFN-γ stimulated Macrophages[J]. Zhen Dong. et al. IMMUNOLOGY. 2020.

Myeloid-specific SIRT1 deletion exacerbates airway inflammatory response in a mouse model of allergic asthma[J]. Tianwen Lai. et al. Aging-US. 2021.

Corilagin suppresses RANKL‐induced osteoclastogenesis and inhibits oestrogen deficiency‐induced bone loss via the NF‐κB and PI3K/AKT signalling pathways[J]. Jinwei Lu. et al. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2020.

Identification of compound D2923 as a novel anti-tumor agent targeting CSF1R[J]. Ying-Qiang Liu. et al. Acta Pharmacologica Sinica. 2018.

The prevention of latanoprost on osteoclastgenesis in vitro and lipopolysaccharide‐induced murine calvaria osteolysis in vivo[J]. Xing Xu. et al. Journal of Cellular Biochemistry. 2017.

 

FOR RESEARCH USE ONLY