Recombinant Human LIF

价格： ￥840 - 2520

品牌：近岸蛋白(Novoprotein) 品牌认证

货号：C017

产品详情

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英文名：Recombinant Human LIF

规格：10ug/50ug/500ug/1mg

Recombinant Human LIF (C017)

产品说明(Description)

Recombinant Human Leukemia Inhibitory Factor is produced by our E.coli expression system and the target gene encoding Ser23-Phe202 is expressed.
Accession #: P15018
Known as: Leukemia Inhibitory Factor; LIF; Differentiation-Stimulating Factor; D Factor; Melanoma-Derived LPL Inhibitor; MLPLI; Emfilermin; LIF; HILDA

制剂（Formulation）
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

质量控制（Quality Control）
Purity:      Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin:   Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
Bioactivity:  Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells.The ED50 for this effect is 25-150 pg/ml.

复溶（Reconstitution）
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存（Storage）
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

背景（Background）
Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.

电泳(SDS-PAGE)

参考文献(Reference)
A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation[J]. Chao Sheng. et al. Nature Communications. 2018.
Determining Osteogenic Differentiation Efficacy of Pluripotent Stem Cells by Telomerase Activity[J]. Siqi Zhang. et al. Tissue Engineering and Regenerative Medicine. 2018.
Isolation of lung multipotent stem cells using a novel microfluidic magnetic activated cell sorting system[J]. L Zeng. et al. Cell Biology International. 2017.
Effects of estrogen on growth and smooth muscle differentiation of vascular wall-resident CD34+ stem/progenitor cells[J]. Wu Y. et al. Atherosclerosis. 2015.
Effect of leukemia inhibitory factor on corneal nerve regeneration of rabbit eyes after laser in situ keratomileusis[J]. Shuling Pan. et al. Neuroscience Letters. 2011.

FOR RESEARCH USE ONLY