Recombinant Human IL-13 (C-6His)

英文名 ：Recombinant Human IL-13 (C-6His)

规格 ：10ug/50ug/500ug/1mg

Recombinant Human IL-13 (C-6His) (CC89)

产品说明(Description)

Recombinant Human Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Gly35-Asn146 is expressed with a 6His tag at the C-terminus. 

Accession #: AAH96139

Known as: Interleukin-13;IL-13

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

 

质量控制(Quality Control) 

Purity:            Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity:   Measured in a cell proliferation assay using TF-1 human erythroleukemic cells

                       The ED50 for this effect is 1.5-4.5 ng/ml.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. 

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.  
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin-13 is also known as IL-13. It is a protein that in humans is encoded by the IL13 gene. Interleukin-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells.It is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY