Thermo Scientific T4 DNA连接酶和克隆试剂盒

英文名 ：T4 DNA Ligase (5 Weiss U/µL)

库存 ：现货

供应商 ：上海恒斐生物科技有限公司

规格 ：1,000 U

Thermo Scientific T4 DNA连接酶和克隆试剂盒

Thermo Scientific™ T4 DNA连接酶

• 室温下粘性末端连接仅需10分钟
• 提供PEG溶液，显著提高平末端连接效率
• 兼容性好，在Thermo Scientific内切酶、PCR及RT缓冲液中均有活性
• 可提供高浓度、低浓度选择，满足不同应用

Thermo Scientific™ 克隆试剂盒

• Rapid DNA连接试剂盒（K1422/K1423），可在5分钟内完成粘性末端或平末端的连接
• CloneJET PCR克隆试剂盒（K1231/K1232），其含有阳性筛选载体，无论DNA片段为粘性末端还是平末端，均可在5-10分钟内完成克隆，且克隆效率高达99%

货号	产品名称	规格	目录价
EL0014	T4 DNA Ligase (5 Weiss U/µL)	200 U	¥278
EL0011	T4 DNA Ligase (5 Weiss U/µL)	1,000 U	¥695
EL0012	T4 DNA Ligase (5 Weiss U/µL)	5 x 1,000 U	¥2,780
EL0013	T4 DNA Ligase, HC (30 U/µL)	5000 U	¥2,800
EL0016	T4 DNA Ligase, LC (1 U/µL)	2x500 U	¥701
K1422	Rapid DNA Ligation Kit	50 rxns	¥1,541
K1423	Rapid DNA Ligation Kit	150 rxns	¥2,975
K1231	CloneJET PCR Cloning Kit	20 rxns	¥1,487
K1232	CloneJET PCR Cloning Kit	40 rxns	¥2,785

描述

Thermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA, or DNA/RNA hybrids. It also joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids.

T4 DNA Ligase requires ATP as a cofactor.

Highlights

• Active in Themo Scientific restriction enzyme, PCR, and RT buffers (when supplemented with ATP)
• Fast—sticky-end ligation is completed in 10 minutes at room temperature
• Supplied with PEG solution for efficient blunt-end ligation

Applications

• Cloning of restriction enzyme generated DNA fragments
• Cloning of PCR products
• Joining of double-stranded oligonucleotide linkers or adaptors to DNA
• Site-directed mutagenesis
• Amplified fragment length polymorphism (AFLP)
• Ligase-mediated RNA detection (see Reference 3)
• Nick repair in duplex DNA, RNA or DNA/RNA hybrids
• Self-circularization of linear DNA.

Includes

• T4 DNA Ligase
• 10X T4 DNA Ligase Buffer
• 50% PEG Solution

Notes

• Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gels. To avoid this, incubate samples with 6X DNA Loading Dye & SDS Solution at 70°C for 5 min or 65°C for 10 minutes and chill on ice prior to electrophoresis.
• The volume of the ligation reaction mixture should not exceed 10% of the competent cell volume in the transformation process.
• Prior to electro-transformation, remove T4 DNA Ligase from the ligation mixture using spin columns or chloroform extraction. The extracted DNA can be further precipitated with ethanol.