Thermo Scientific™ 直接PCR试剂

保存条件 ：-20

英文名 ：Phire Tissue Direct PCR Master Mix

库存 ：现货

供应商 ：上海恒斐生物科技有限公司

CAS号 ：Thermo Scientific™ 直接PCR试剂 无需任何纯化步骤，只需微量的原始样本直接作为PCR反应的模板 已针对多种样本类型进行过测试 两种直接PCR方案，满足不同实验需求

规格 ：250 reactions

Thermo Scientific™ 直接PCR试剂

货号	产品名称	规格	目录价
F170S	Phire Tissue Direct PCR Master Mix	250 x 20 µL rxns	¥1,602
F170L	Phire Tissue Direct PCR Master Mix	1,250 x 20 µL rxns	¥6,770
F160S	Phire Plant Direct PCR Master Mix	250 x 20 µL rxns	¥1,292
F160L	Phire Plant Direct PCR Master Mix	1,250 x 20 µL rxns	¥5,614
F175S	Phusion Blood Direct PCR Master Mix	100 x 20 µL rxns	¥989
F175L	Phusion Blood Direct PCR Master Mix	500 x 20 µL rxns	¥3,898

 

描述

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects. The Master Mix containing Phire Hot Start II DNA Polymerase is specially formulated to perform PCR in the presence of different animal tissue-derived inhibitors such as collagen, melanin and eumelanin (hair, skin) or myoglobin (muscle). The kit also includes Thermo Scientific DNARelease Additive, which can be used to improve the release of DNA from difficult tissues.

Highlights

• Direct PCR—sample is added directly to PCR reaction, therefore therese is no need for time-consuming and expensive DNA purification steps
• Dilution & Storage protocol available—allows tens of PCR reactions from one tiny sample and allows re-testing
• Specially engineered Phire DNA polymerase—extremely short PCR protocol times
• Master Mix format with premixed gel loading dye—minimizes possibility of cross-contamination, reduces sample handling and allows direct loading on gel

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

The optimal annealing temperature for Phire DNA Polymerases may differ significantly from that of Taq-based polymerases.

For optimal results start by accurately calculating your Tm with our Tm calculator.

规格

Includes Label or Dye:	Yes
Polymerase:	Phire Hot Start II DNA polymerase
Hot Start:	Built-In Hot Start
GC-Rich PCR Performance:	Medium
Reaction Format:	SuperMix or Master Mix
Product Overhang:	Blunt
Reaction Speed:	Fast
Product Line:	Phire
Product Size:	250 reactions

内容及储存

Contains: 
• 2 x 1.25 mL Phire Tissue Direct PCR Master Mix (2X) 
• 5 mL Dilution Buffer 
• 3 x 100 µL DNARelease Additive 
• 40 µL Control Primer Mix (25 µM each) 
• 2 x 1.25 mL Water, nuclease- free 
• 50 µg O'GeneRuler Express DNA Ladder (sufficient for 100 applications)

Store at -20°C.