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保质期 :6个月以上
抗体英文名 :Mouse Anti-Human TNF-alpha Monoclonal Antibody
是否单克隆 :1
抗原来源 :人
级别 :RUO
标记物 :无
供应商 :PeproTech(派普泰克)
抗原 :TNF-alpha
宿主 :小鼠
保存条件 :-20oC
应用范围 :ELISA/WB/IHC/中和(详见说明书)
适应物种 :人
目录编号 :500-M26
库存 :大量
Preparation : |
Produced in BALB/c mice using highly pure (>98%) recombinant human TNF-alpha as the immunizing antigen. This IgG1K antibody was purified from cell culture by Protein G affinity chromatography. |
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Catalog #: |
500-M26 |
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Source : |
Mice |
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Formulation : |
A sterile filtered antibody solution was lyophilized from PBS. |
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Stability : |
The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20o C. Frozen aliquots are stable for at least six weeks at 2-8o C or for at least six months at -20o C. Avoid repeated freeze-thaw cycles. |
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Reconstitution: |
Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 1.0 mg/ml. |
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Applications : |
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Selected References : |
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Neutralization |
To yield one-half maximal inhibition [ND 50 ] of the biological activity of hTNF-α (0.5 ng/ml), a concentration of < 0.1 μg/ml of this antibody is required. |
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Elisa |
In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human TNF-α when used with 's biotinylated antigen affinity purified anti-human TNF-α (500-P31ABt) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml. |
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Western Blot |
To detect hTNF-α by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTNF-α is 0.5-1.0 ng/lane, under reducing conditions and 0.25-0.50 ng/lane, under non-reducing conditions. |
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Country of Origin : |
USA |
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Special Sizes: |
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Usage: |
派普泰克生物科技(苏州)有限公司(PeproTech中国)
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入驻年限:15年