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英文名 :MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate
供应商 :祥生兴业
规格 :2 x 96 Tests
MassChrom® Free Metanephrines in Plasma with 96 SPE Well Plate
3-甲氧酪胺 变肾上腺素 去甲变肾上腺素- 3-Methoxytyramine
- Metanephrine
- Normetanephrine
6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
Isotopically labelled internal standard for each analyte
Fully automated workflow solution available
Description
MassChrom® Free Metanephrines in Plasma
With 96 SPE Well Plates, suitable for manual and automated sample preparation
For 2 x 96 determinations
Clinical relevance
This kit allows the quantitative determination of metanephrine, normetanephrine and 3-methoxytyramine in plasma by LC-MS/MS.
One typical application for this kit is the analysis of patient samples with a suspicion of pheochromocytomas. These tumours and other neural crest tumours (e.g. paragangliomas and neuroblastomas) secrete metanephrine, normetanephrine and 3-methoxytyramine. The plasma free metanephrines are more stable and secreted more evenly than catecholamines (epinephrine, norepinephrine, and dopamine). Some studies show that the analysis of plasma free metanephrines has the highest sensitivity and specificity in pheochromocytoma diagnostics compared to plasma free catecholamines and metanephrines in urine.
Product advantages
- 6PLUS1® Multilevel Calibrator Set and MassCheck® controls based on human plasma
- Simple sample prep in just a few steps
- Chromatographic separation of analytes – no co-elution
- Isotopically labelled internal standards for all analytes
- Fully automated workflow solution available
This kit encompasses all required components and allows a simple and fast sample preparation: solid phase extraction (SPE) without equilibration/conditioning steps, analyte enrichment without evaporation step, only one elution step. The analytes are chromatographically separated to avoid co-elution and to minimise risks of interferences. The use of stable isotope-labelled internal standards for every single analyte ensures reproducible and reliable quantification. The 6PLUS1® Multilevel Plasma Calibrator Set and MassCheck® controls ensure high precision of results. They are obtained from human plasma to minimise matrix-induced differences.
A fully automated workflow solution is also available.
Alternatively, there is also a method available using Sample Clean Up Columns (81000/C).
Chromatogram
Technical Data
Method of Analysis
Number of Tests
Parameter
Analysis Time
Linearity
Metanephrine: up to 5650 ng/l
Normetanephrine: up to 8500 ng/l
3-Methoxytyramine: up to 5800 ng/l
Limit of quantification
Metanephrine: 15 ng/l
Normetanephrine: 7 ng/l
3-Methoxytyramine: 11 ng/l
Intraassay
Interassay
Recovery
Gradient
Specimen
Sample Preparation
- Pipette 500 µl Dilution Buffer and 25 µl Internal Standard Mix into each well of the 96 SPE Well Plate.
- Add 500 µl of the sample/calibrator/MassCheck® control, centrifuge, discard effluent.
- Add 0.9 ml Wash Buffer 1, centrifuge, discard effluent.
- Add 2 x 0.9 ml Wash Buffer 2, centrifuge, discard effluent.
- Add 250 µl Elution Buffer into each well and centrifuge.
- Inject up to 25 µl.
Injection Volume
Ionisation
MS/MS-Mode
Please note
北京祥生兴业科技有限公司
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入驻年限:10年