AmpliCap™ SP6 High Yield Message Maker Kit | C-AC0706 | CELLSCRIPT

The AmpliCap™ SP6 High Yield Message Maker Kit produces capped RNA by in vitro transcription using SP6 RNA polymerase and the standard cap analog m7G[5']ppp[5']G. A 2 hour, 20 μl AmpliCap reaction yields up to 35 μg of m7G-capped RNA from 1 μg of the 1.4 kb standard SP6 control template DNA. The m7G[5']ppp[5']G cap analog/NTP PreMix contains all four ribonucleotides and the monomethylated cap analog. The PreMix ensures the optimal concentration of each NTP and ratio of methylated cap analog to GTP (4:1), maximizing transcript capping (~80%) and yield. Because the concentration of GTP in the reaction is limiting, the cap analog is preferentially incorporated as the first or 5'-terminal G of the transcript. CELLSRIPT also offers the AmpliCap-Max™ T7 High Yield Message Maker Kit and the MessageMAX™ T7 ARCA-Capped Message Transcription Kit, both of which produce unsurpassed yields of capped in vitro transcripts (up to 60 μg of RNA in a 30 minute standard control reaction with an 80% capping efficiency). The AmpliCap-Max Kit uses the standard RNA Cap Analog m7G[5']ppp[5']G and the MessageMAX Kit uses the anti-reverse cap analog m27,3'-OG[5']ppp[5'].

CELLSCRIPT C-AC0706 品牌名称：
CELLSCRIPT

CELLSCRIPT C-AC0706 品牌logo：

CELLSCRIPT专注于再生医学，细胞再生学以及细胞疗法。细胞、组织或器官的移植治疗及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。

The mission of CELLSCRIPT™ is to provide the best products and technologies for making and using RNA for translation in cells for clinical research and therapeutics.

Current products include kits for in vitro transcription, 5' RNA capping using either a cap analog or capping enzymes, and 3' RNA polyadenylation, as well as all-in-one kits for making capped, poly(A)-tailed mRNA for translation in cells.

CELLSCRIPT™ recently introduced INCOGNITO™ RNA Transcription Kits for in vitrosynthesis of RNA that contains modified nucleosides, such as pseudouridine (Ψ) and/or 5-methylcytidine (m5C) in place of the corresponding U or C canonical nucleosides (Figure 1). These kits are so-named because the capped, polyadenylated and nucleoside-modified RNA products (called "INCOGNITO mRNAs") are disguised so they do not induce innate immune responses to the same extent as the corresponding unmodified mRNAs when transfected into mammalian cells that express a variety of RNA sensors.