MessageMAX™ T7 ARCA-Capped Message | C-MMA60710 | CELLSCRIPT
价格: ¥5548
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保存条件 :-20度
供应商 :安诺伦(北京)生物科技有限公司
The MessageMAX™ T7 ARCA-Capped Message Transcription Kit produces capped RNA by in vitro transcription using T7 RNA polymerase and the anti-reverse cap analog (ARCA) m27,3'-OG[5']ppp[5']. A 30 minute, 20 μl MessageMAX reaction yields up to 60 μg of ARCA-capped RNA from 1 μg of the 1.4 kb standard T7 control template DNA. Because ARCA contains a 3'-O-methyl group on the m7G nucleotide (Figure 1), ARCA can only be incorporated in the correct orientation at the 5' end of the RNA during an in vitro transcription/capping reaction.1-3 This is not true for the standard cap analog (m7G[5']ppp[5']G). Thus, ARCA incorporation results in the synthesis of capped RNA that is more efficiently translated in vivo than standard cap analog. The ARCA/NTP PreMix contains all four ribonucleotides and the ARCA. The PreMix ensures the optimal concentration of each NTP and ratio of ARCA to GTP (4:1), maximizing transcript capping (~80%) and yield. Because the concentration of GTP in the reaction is limiting, the ARCA is preferentially incorporated as the first or 5'-terminal G of the transcript. CELLSCRIPT also offers the INCOGNITO™ T7 ARCA 5mC- & -RNA Transcription Kit for the production of 5-methylcytosine- pseudouridine-containing (GA m5C) ARCA-capped RNA. Such RNA has been used in the landmark publication by Warren et al. (2010), to successfully reprogram multiple human cell types into induced pluripotent stem cells.4 It has been shown that -mRNAs and 5mCmRNAs are translated into protein at higher levels and induce lower innate immune responses in human and other mammalian cells that express various RNA sensors compared to corresponding unmodified mRNAs.
CELLSCRIPT C-MMA60710 品牌名称:
CELLSCRIPT
CELLSCRIPT C-MMA60710 品牌logo:
CELLSCRIPT C-MMA60710 品牌官网:
http://www.cellscript.com
CELLSCRIPT C-MMA60710 品牌介绍:
CELLSCRIPT专注于再生医学,细胞再生学以及细胞疗法。细胞、组织或器官的移植治疗及修复中应用的细胞或生物体。CellScript还生产用于传递和翻译的合成修饰mRNA。
The mission of CELLSCRIPT™ is to provide the best products and technologies for making and using RNA for translation in cells for clinical research and therapeutics.
Current products include kits for in vitro transcription, 5' RNA capping using either a cap analog or capping enzymes, and 3' RNA polyadenylation, as well as all-in-one kits for making capped, poly(A)-tailed mRNA for translation in cells.
CELLSCRIPT™ recently introduced INCOGNITO™ RNA Transcription Kits for in vitrosynthesis of RNA that contains modified nucleosides, such as pseudouridine (Ψ) and/or 5-methylcytidine (m5C) in place of the corresponding U or C canonical nucleosides (Figure 1). These kits are so-named because the capped, polyadenylated and nucleoside-modified RNA products (called "INCOGNITO mRNAs") are disguised so they do not induce innate immune responses to the same extent as the corresponding unmodified mRNAs when transfected into mammalian cells that express a variety of RNA sensors.
The mission of CELLSCRIPT™ is to provide the best products and technologies for making and using RNA for translation in cells for clinical research and therapeutics.
Current products include kits for in vitro transcription, 5' RNA capping using either a cap analog or capping enzymes, and 3' RNA polyadenylation, as well as all-in-one kits for making capped, poly(A)-tailed mRNA for translation in cells.
CELLSCRIPT™ recently introduced INCOGNITO™ RNA Transcription Kits for in vitrosynthesis of RNA that contains modified nucleosides, such as pseudouridine (Ψ) and/or 5-methylcytidine (m5C) in place of the corresponding U or C canonical nucleosides (Figure 1). These kits are so-named because the capped, polyadenylated and nucleoside-modified RNA products (called "INCOGNITO mRNAs") are disguised so they do not induce innate immune responses to the same extent as the corresponding unmodified mRNAs when transfected into mammalian cells that express a variety of RNA sensors.
No. | 品牌 | 货号 | 名称 | 规格 |
1 | CELLSCRIPT | C-ACM04037 | AmpliCap-Max™ T7 High Yield Message Maker Kit | 25 rxns |
2 | CELLSCRIPT | C-AC0706 | AmpliCap™ SP6 High Yield Message Maker Kit | 25 rxns |
3 | CELLSCRIPT | C-C50110 | Anti-Reverse Cap Analog (ARCA) | 500 nmol |
4 | CELLSCRIPT | C-CC15011H | Cap-Clip™ Acid Pyrophosphatase | 100 U |
5 | CELLSCRIPT | C-ICSY110510 | INCOGNITO™ SP6 Ψ-RNA Transcription Kit | 10 rxns |
6 | CELLSCRIPT | C-MSC100625 | T7 mScript™ Standard mRNA Production System | 25 rxns |
7 | CELLSCRIPT | C-MMA60710 | MessageMAX™ T7 ARCA-Capped Message Transcription Kit | 10 rxns |
8 | CELLSCRIPT | C-SRI6310K | ScriptGuard™ RNase Inhibitor | 10,000 U |
9 | CELLSCRIPT | C-SCMT0610 | ScriptCap™ 2'-O-Methyltransferase Kit | 10 rxns |
10 | CELLSCRIPT | C-ICTY110510 | INCOGNITO™ T7 Ψ-RNA Transcription Kit | 10 rxns |
11 | CELLSCRIPT | C-MMA60710 | MessageMAX™ T7 ARCA-Capped Message Transcription Kit | 10 rxns |
12 | CELLSCRIPT | C-C61025 | Monomethylated RNA Cap Analog | 1,250 nmol |
13 | CELLSCRIPT | C-PRM101125 | PseudoU-RNA Molecular Weight Markers | 25 lanes |
14 | CELLSCRIPT | C-AS3106 | SP6-Scribe™ Standard RNA IVT Kit | 50 rxns |
15 | CELLSCRIPT | C-PAP5104H | A-Plus™ Poly(A) Polymerase Tailing Kit | 50 rxns |
CELLSCRIPT C-MMA60710 联系方式:
联系人:谢先生
400电话:4009658633
办公电话:010-56256916
QQ:3212874516,3198592576
网址:http://www.annoron.com/
CELLSCRIPT C-MMA60710 产品相关链接:
1 ADP P/N 384
2 Collagen P/N 385
3 Thrombin P/N 386
4 Arachidonic Acid P/N 390
5 Epinephrine P/N 393
安诺伦(北京)生物科技有限公司
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入驻年限:9年