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保存条件 ：-20℃
保质期 ：半年
英文名 ：pHIS2
库存 ：200
供应商 ：沪震生物
规格 ：5μg
pHIS2
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0421 | pHIS2 | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | TRP1 ,T7,T3 |
|---|---|
| 复制子: | pUC ori，FI ori |
| 质粒分类: | 酵母系列，酵母单杂交载体 |
| 质粒大小: | 7207bp |
| 原核抗性: | Kan |
| 筛选标记: | TRP1 |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧 LB |
| 表达宿主: | 酵母细胞 |
| 5'测序引物: | M13F:TGTAAAACGACGGCCAGT |
| 3'测序引物: | 根据序列设计引物 |
质粒简介
pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker™ One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene,located downstream of a multiple cloning site (MCS) and the minimal promoter of the HIS3 locus(PminHIS3). Cis-acting DNA sequences, or DNA target elements, can be inserted into the MCS andused as baits to screen GAL4 AD/cDNA fusion libraries for proteins that interact with the targetsequence. A protein-DNA (or one-hybrid) interaction can be detected by performing the assay in ayeast strain such as Y187 that is auxotrophic for histidine. Positive one-hybrid interactions driveexpression of the HIS3 reporter gene, which enables the host cell to grow on histidine-deficientmedia.
质粒图谱
上海沪震实业有限公司
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入驻年限：11年
