载体pABAI

价格： ¥800 - 1380

品牌：HZbscience

货号：HZ0232

产品详情

pABAI

产品信息

产品货号	产品名称	产品规格	优惠价
HZ0232	pABAI	20μl	¥请询价

使用说明

沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

基本信息

启动子:	URA3
复制子:	ori
质粒分类:	酵母系列，酵母单杂交载体
质粒大小:	4894bp
原核抗性:	Amp
筛选标记:	URA3
克隆菌株:	DH5α
培养条件:	37℃，有氧 LB
表达宿主:	酵母细胞
5'测序引物:	根据序列设计引物
3'测序引物:	根据序列设计引物

质粒简介

pAbAi is a yeast reporter vector that can be used in one-hybrid assays to identify and characterize DNA-binding proteins. The vector, which was specifically designed for use with the Matchmaker™ Gold Yeast One-Hybrid Library Screening System (Cat. No. 630491), contains a multiple cloning site (MCS) upstream of the yeast iso-1-cytochrome C minimal promoter(not shown) and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A (AbA; Cat. No. 630466). A cis-acting element (i.e., a target sequence) can be inserted into the MCS and used as bait to screen GAL4 AD/cDNA fusion libraries (e.g. Mate & Plate™ Libraries) for proteins that interact with the target sequence. Positive protein-DNA (i.e.,one-hybrid) interactions drive the expression of AUR1-C. As a result, one-hybrid interactions can be detected by selecting for yeast that are resistant to AbA.

pAbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the ura3-52 locus of the yeast strain provided in theMatchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for selection of recombinant yeast (1, 2). The vector also contains a Col E1 origin of replication and an ampicillin resistance gene (Ampr) for propagation and selection in E.coli.