载体pGreen-Puro

价格： ¥800 - 1380

品牌：HZbscience

货号：HZ0420

产品详情

pGreen-Puro

产品信息

产品货号	产品名称	产品规格	优惠价
HZ0420	pGreen-Puro		¥

使用说明

沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

基本信息

启动子:	CMV
复制子:	pUC ori
终止子:	SV40 poly(A) signal
质粒分类:	病毒系列，慢病毒克隆载体
质粒大小:	7.8kb
原核抗性:	Amp
筛选标记:	Puro
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	CMV-F：CGCAAATGGGCGGTAGGCGTG
3'测序引物:	根据序列设计引物

质粒简介

The pGreenPuro™ Vector is designed to express a single-stranded shRNA sequence with a fold-back stem-loop structure (also known as a “hairpin”) from a RNA polymerase III H1 promoter .The hairpin-type siRNA (shRNA) template oligonucleotides need to be cloned into unique BamHI/EcoRI sites located just downstream of an H1 promoter. The pGreenPuro™ vector needs to be linearized by restriction digest with BamHI and EcoRI, and purified to remove the stuffer fragment. When linearized, the vector contains two unique 5’ overhangs to facilitate directional cloning of shRNA template oligos with minimal self-ligation background . When the shRNA construct is expressed from constitutive H1 promoter and terminated with the TTTTT sequence, the shRNA transcript folds into the hairpin structure, which is recognized by the DICER enzyme, cleaved to form a functional ds siRNA and transferred to a RISC complex for selective digestion of complementary target mRNAs .Two PCR primers are designed for regions flanking the shRNA insert in order to provide a simple way for screening of plasmid clones for the